A juvenile hormone binding protein from the house fly and its possible relationship to insecticide resistance

High-affinity juvenile hormone 1 (JH) binding to protein was demonstrated in house fly adults and larvae. Techniques were developed to optimize isolation of the protein and measurement of binding kinetics. Resistant flies showed a significant decrease in JH binding site numbers and a significant inc...

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Bibliographic Details
Published in:Archives of insect biochemistry and physiology 1998, Vol.37 (1), p.64-72
Main Authors: Plapp, F.W. Jr, Carino, F.A, Wei, V.K
Format: Article
Language:English
Subjects:
AMINO ACID SEQUENCES
BINDING PROTEINS
BINDING SITES
CHEMICAL COMPOSITION
CHROMOSOME
CHROMOSOMES
COMPOSICION QUIMICA
COMPOSITION CHIMIQUE
CONTROL DE INSECTOS
CROMOSOMAS
GENETIC INHERITANCE
HEREDITE
HERENCIA GENETICA
HORMONAS JUVENILES
HORMONE JUVENILE
INSECT CONTROL
INSECTICIDAS
INSECTICIDE
insecticide resistance
INSECTICIDES
JH binding kinetics
JH binding protein
juvenile hormone
JUVENILE HORMONES
LUTTE ANTIINSECTE
METABOLIC RESISTANCE
MUSCA DOMESTICA
PROTEINAS AGLUTINANTES
PROTEINE DE LIAISON
PURIFICACION
PURIFICATION
RESISTANCE AUX PRODUITS CHIMIQUES
RESISTANCE TO CHEMICALS
RESISTENCIA A PRODUCTOS QUIMICOS
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Description
Summary:High-affinity juvenile hormone 1 (JH) binding to protein was demonstrated in house fly adults and larvae. Techniques were developed to optimize isolation of the protein and measurement of binding kinetics. Resistant flies showed a significant decrease in JH binding site numbers and a significant increase in JH binding site numbers compared to susceptibles. Genetic tests showed these changes mapped to chromosomes 5 and 2, respectively, the chromosomes that play a major role in metabolic resistance to insecticides. With larvae, purification of the binding protein was achieved with photolysis in combination with ammonium sulfate fractionation of cytosols. The purified protein had a molecular weight of ca. 22 kDa and occurred as a dimer. The 12 N-terminal amino acids were identified. Possible relationships of JH binding proteins and metabolic resistance to insecticides are discussed
ISSN:0739-4462
1520-6327
DOI:10.1002/(SICI)1520-6327(1998)37:1<64::AID-ARCH8>3.0.CO;2-V