Surfactant stimulation of growth in the nematode Caenorhabditis elegans

Size fractionation has been used to isolate L1 L2 larvae from mixed cultures of the nematode, Caenorhabditis elegans. Worm lengths have been compared during growth in synchronized liquid and agar cultures. Supplementation of liquid S medium with 10 ppm surfactant (Pluronics F-68, F-127, F-38, L-35;...

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Bibliographic Details
Published in:Enzyme and microbial technology 1997-05, Vol.20 (6), p.462-470
Main Authors: Mutwakil, Mohammed H.A.Z., Steele, Tamazin J.G., Lowe, Kenneth C., de Pomerai, David I.
Format: Article
Language:English
Subjects:
amino acid incorporation
Biochemistry. Physiology. Immunology. Molecular biology
Biological and medical sciences
Biotechnology
Caenorhabditis elegans
Fundamental and applied biological sciences. Psychology
growth
Invertebrates
Methods. Procedures. Technologies
Nemathelminthia. Plathelmintha
nematode
Others
Physiology. Development
size separation
stress response
Surfactants
synchronous culture
transgenic strain
Various methods and equipments
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Summary:Size fractionation has been used to isolate L1 L2 larvae from mixed cultures of the nematode, Caenorhabditis elegans. Worm lengths have been compared during growth in synchronized liquid and agar cultures. Supplementation of liquid S medium with 10 ppm surfactant (Pluronics F-68, F-127, F-38, L-35; Tween-20 or Triton X-100) promoted a significant stimulation of growth over three days in all cases. Because of possible poor nutrition and/or aeration in liquid culture, experiments were repeated using the same surfactants in standard NGM agar. Four surfactants again stimulated worm growth significantly whereas two (Tween-20 and Pluronic F-68) did not. Differences between these surfactants were also demonstrated with respect to: (i) toxicity; (ii) induction of stress responses in a transgenic hsp/reporter strain; and (iii) stimulation of amino acid incorporation into soluble protein both initially and after 41 h of surfactant treatment. These surfactants, and in particular, Pluronic F-127, are potentially valuable as culture supplements for enhancing nematode larval growth. Possible mechanisms for growth promotion by surfactants are discussed in light of the fixed somatic cell lineage and the fact that Pluronic F-127 did not speed up maturation from L4 larvae into egg-bearing adults.
ISSN:0141-0229
1879-0909
DOI:10.1016/S0141-0229(96)01173-8