Long-term high immune response to diphtheria toxoid in rodents with diphtheria toxoid conjugated to dextran as a single contact point delivery system

Cross-linked dextran beads were used as a carrier for development of a ‘single-contact’ vaccine delivery system. Diphtheria toxoid (DT) was covalently coupled to dextran beads (DEX-DT conjugate). The conjugate was immunoreactive with antibodies raised against native DT. Immunization of rats with DEX...

Full description

Saved in:
Bibliographic Details
Published in:Vaccine 1997-12, Vol.15 (17), p.1867-1871
Main Authors: Diwan, M., Misra, A., Khar, R.K., Talwar, G.P.
Format: Article
Language:English
Subjects:
Animals
Antibodies, Bacterial - biosynthesis
Bacteriology
Biological and medical sciences
Chemical Phenomena
Chemistry, Physical
cross-linked dextran beads
Dextrans - administration & dosage
Dextrans - immunology
Dextrans - toxicity
Diphtheria Toxoid - administration & dosage
Diphtheria Toxoid - immunology
Diphtheria Toxoid - toxicity
diphtheria toxoid immunoreactivity
Drug Delivery Systems
Female
Fundamental and applied biological sciences. Psychology
Male
Microbiology
Microspheres
Particle Size
Rats
Rats, Wistar
Time Factors
Vaccine delivery
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies
Vaccines, Synthetic - administration & dosage
Vaccines, Synthetic - immunology
Vaccines, Synthetic - toxicity
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Cross-linked dextran beads were used as a carrier for development of a ‘single-contact’ vaccine delivery system. Diphtheria toxoid (DT) was covalently coupled to dextran beads (DEX-DT conjugate). The conjugate was immunoreactive with antibodies raised against native DT. Immunization of rats with DEX-DT conjugate generated on average 24 times higher antibody titres against diphtheria toxoid than immunization with the conventional DT absorbed on alum. The immune response was sustained for 9 months, with gradual decline of antibody titres thereafter. Significant antibody titres were measurable in rats after 1 year of immunization. DEX-DT had neither acute nor long-term adverse effects on the health of animals, as evidenced by local reactions, general behaviour, food intake, body weight gain and survival compared with controls.
ISSN:0264-410X
1873-2518
DOI:10.1016/S0264-410X(97)00137-0