Rational attenuation of Corynebacterium pseudotuberculosis: potential cheesy gland vaccine and live delivery vehicle

The phospholipase D (PLD) gene (pld) has been deleted from the Corynebacterium pseudotuberculosis chromosome by using site-specific mutagenesis. Sheep infection trials indicate that the PLD-negative C. pseudotuberculosis strain (Toxminus) is incapable of inducing caseous lymphadenitis (cheesy gland)...

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Bibliographic Details
Published in:Infection and Immunity 1992-07, Vol.60 (7), p.2900-2905
Main Authors: Hodgson, A.L.M. (CSIRO Division of Animal Health, Victoria, Australia), Krywult, J, Corner, L.A, Rothel, J.S, Radford, A.J
Format: Article
Language:English
Subjects:
Animals
Antibody Formation
Bacteriology
Biological and medical sciences
Chromosome Mapping
Corynebacterium Infections - immunology
Corynebacterium Infections - prevention & control
CORYNEBACTERIUM PSEUDOTUBERCULOSIS
Corynebacterium pseudotuberculosis - pathogenicity
Dose-Response Relationship, Immunologic
ESTERASAS
ESTERASE
Fundamental and applied biological sciences. Psychology
GENE
GENES
IMMUNITE CELLULAIRE
IMMUNITE HUMORALE
Immunization, Passive
INMUNIDAD CELULAR
INMUNIDAD HUMORAL
Interferon-gamma - biosynthesis
LINFOADENITIS
LYMPHADENITE
Microbiology
Mutagenesis, Insertional
Mutagenesis, Site-Directed
OVIN
OVINOS
Phospholipase D - genetics
Phospholipase D - immunology
Plasmids
Sheep
Time Factors
VACCIN SYNTHETIQUE
VACCIN VIVANT
VACCINATION
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies
Vaccines, Attenuated - biosynthesis
VACUNA SINTETICA
VACUNA VIVA
VACUNACION
Virulence - genetics
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Description
Summary:The phospholipase D (PLD) gene (pld) has been deleted from the Corynebacterium pseudotuberculosis chromosome by using site-specific mutagenesis. Sheep infection trials indicate that the PLD-negative C. pseudotuberculosis strain (Toxminus) is incapable of inducing caseous lymphadenitis (cheesy gland) even at doses two logs higher than that at which the wild-type strain produces the disease. This clearly establishes PLD as a major C. pseudotuberculosis virulence factor. Vaccination of sheep with live Toxminus C. pseudotuberculosis elicits strong humoral and cell-mediated immune responses and protects the animals from wild-type challenge
ISSN:0019-9567
1098-5522
DOI:10.1128/iai.60.7.2900-2905.1992