Cloning and nucleotide sequence of a thermostable cyclodextrin glycosyltransferase gene from Thermoanaerobacter sp. ATCC 53627 and its expression in Escherichia coli

A gene, cgtA, encoding an extremely thermostable cyclodextrin glycosyltransferase (CGTase) was cloned from a thermophilic anaerobe, Thermoanaerobacter sp. ATCC 53627 and expressed in Escherichia coli. DNA and protein sequencing revealed that the mature enzyme of 683 amino acid residues (MW 75 kDa) w...

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Bibliographic Details
Published in:Biotechnology letters 1997-10, Vol.19 (10), p.1027-1031
Main Authors: Jorgensen, S.T, Tangney, M, Starnes, R.L, Amemiya, K, Jorgensen, P.L
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biological Sciences
Biotechnology
Diverse techniques
food microbiology
food processing
Fundamental and applied biological sciences. Psychology
genbank/z35484
Genetic engineering
Genetic technics
Methods. Procedures. Technologies
Molecular and cellular biology
molecular sequence data
Synthetic digonucleotides and genes. Sequencing
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Summary:A gene, cgtA, encoding an extremely thermostable cyclodextrin glycosyltransferase (CGTase) was cloned from a thermophilic anaerobe, Thermoanaerobacter sp. ATCC 53627 and expressed in Escherichia coli. DNA and protein sequencing revealed that the mature enzyme of 683 amino acid residues (MW 75 kDa) was preceded by a signal peptide of 27 amino acid residues. The sequence of the Thermoanaerobacter CGTase was similar to sequences of Bacillus CGTases, with more than 58% identity, and very similar (89% identity) to a CGTase enzyme from Thermoanaerobacterium thermosulfurogenes.
ISSN:0141-5492
1573-6776
DOI:10.1023/A:1018459703742