Effect of dilution rate on the stability of TOL plasmid pTK0 in Pseudomonas putida PPK1

Continuous culture fermentation of Pseudomonas putida PPK1, containing the naturally occurring TOL plasmid pTK0, was carried out in either benzoate- or succinate-limited chemostats. The apparent stability of the plasmid decreased with increasing the dilution rate. An established model was used to pr...

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Bibliographic Details
Published in:Biotechnology letters 1997-10, Vol.19 (10), p.985-988
Main Authors: KARBASI, M, KESHAVARZ, T
Format: Article
Language:English
Subjects:
Bacteriology
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
Fermentation
Fundamental and applied biological sciences. Psychology
Genetics
Microbiology
Mission oriented research
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Summary:Continuous culture fermentation of Pseudomonas putida PPK1, containing the naturally occurring TOL plasmid pTK0, was carried out in either benzoate- or succinate-limited chemostats. The apparent stability of the plasmid decreased with increasing the dilution rate. An established model was used to predict the contribution of segregational instability and growth rate difference to the apparent plasmid stability.[PUBLICATION ABSTRACT]
ISSN:0141-5492
1573-6776
DOI:10.1023/A:1018491116037