Production and characterization of monoclonal antibodies to budgerigar fledgling disease virus major capsid protein VP sub(1)

Eleven hybridoma cell lines producing monoclonal antibodies (MAbs) against intact budgerigar fledgling disease (BFD) virions were produced and characterized. These antibodies were selected for their ability to react with BFD virions in an enzyme-linked immunosorbent assay. Each of these antibodies w...

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Bibliographic Details
Published in:Avian diseases 1992-01, Vol.36 (3), p.543-553
Main Authors: Fattaey, A, Lenz, L, Consigli, R A
Format: Article
Language:English
Subjects:
budgerigar fledgling disease virus
Melopsittacus undulatus
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Summary:Eleven hybridoma cell lines producing monoclonal antibodies (MAbs) against intact budgerigar fledgling disease (BFD) virions were produced and characterized. These antibodies were selected for their ability to react with BFD virions in an enzyme-linked immunosorbent assay. Each of these antibodies was reactive in the immunofluorescent detection of BFD virus-infected cells. These antibodies immunoprecipitated intact virions and specifically recognize the major capsid protein, VP sub(1), of the dissociated virion. The MAbs were found to preferentially recognized native BFD virus capsid protein when compared with denatured virus protein. These MAbs were capable of detecting BFD virus protein in chicken embryonated cell-culture lysates by dot-blot analysis.
ISSN:0005-2086