Structure and specific expression of a Nicotiana sylvestris putative amino-acid transporter gene in mature and in vitro germinating pollen

We report here the structure and expression analysis of a Nicotiana sylvestris gene, called nsaap1, coding for a protein which shows significant identity to the members of the Arabidopsis thaliana amino acid permease family. The nsaap1 gene is expressed exclusively in binucleate pollen. Its transcri...

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Bibliographic Details
Published in:Plant molecular biology 1997-12, Vol.35 (6), p.855-864
Main Authors: Lalanne, E. (Paris XI Univ., Orsay (France). Inst. de Biotechnologie des Plantes), Mathieu, C, Roche, O, Vedel, F, Pape, R. De
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino Acid Transport Systems
Amino acids
Cell division
DNA, Complementary - metabolism
EXPRESION GENICA
EXPRESSION DES GENES
Gene Dosage
GENE EXPRESSION
Genes, Plant
GENETICA MOLECULAR
GENETIQUE MOLECULAIRE
Genome, Plant
Germination
Membrane Proteins - genetics
Membrane Proteins - metabolism
Membrane Transport Proteins - chemistry
Membrane Transport Proteins - genetics
Membrane Transport Proteins - metabolism
MOLECULAR GENETICS
Molecular Sequence Data
Nicotiana - enzymology
Nicotiana - genetics
NICOTIANA SYLVESTRIS
Plant biology
Plant Proteins - chemistry
Plant Proteins - genetics
Plant Proteins - metabolism
Plants, Toxic
POLEN
POLLEN
Pollen - enzymology
Pollen - genetics
Pollen - growth & development
Sequence Analysis, DNA
Transcription, Genetic
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Summary:We report here the structure and expression analysis of a Nicotiana sylvestris gene, called nsaap1, coding for a protein which shows significant identity to the members of the Arabidopsis thaliana amino acid permease family. The nsaap1 gene is expressed exclusively in binucleate pollen. Its transcripts begin to accumulate after first pollen mitosis and dramatically increase in mature pollen shortly before anthesis and during in vitro pollen germination. The nsaap1 5'-flanking region contains long regions homologous to the promoter region of the tobacco pollen specific eIF-4A8 translation factor. Our results support the occurrence of an amino acid remobilization in binucleate pollen. The nsaap1 gene could play a role in exchanges between the vegetative and the generative pollen cells.
ISSN:0167-4412
1573-5028
DOI:10.1023/A:1005812419151