Development of a combined selective enrichment method and polymerase chain reaction (PCR) assay for sensitive detection of Salmonella in food samples

PCR assays were formatted using primer pairs homologous to phoE and invA genes. The amplification conditions were optimized with pure cultures and reactions were carried out to define selectivity, specificity and sensitivity of both primer pairs. The performance of the invA primer pair was better th...

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Bibliographic Details
Published in:World journal of microbiology & biotechnology 1997-11, Vol.13 (6), p.649-654
Main Authors: Marsiglia, M.L, Ikuta, N, Fonseca, A.K, Schuch, D.T, Hotzel, I, Ozaki, L.S, Marques, E.K, Lunge, V.R
Format: Article
Language:English
Subjects:
Bacteria
Bacteriological methods and techniques used in bacteriology
Bacteriology
Biological and medical sciences
Food
Food contamination
Food contamination & poisoning
Food industries
Food microbiology
Fundamental and applied biological sciences. Psychology
Microbiology
Polymerase chain reaction
Salmonella
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Summary:PCR assays were formatted using primer pairs homologous to phoE and invA genes. The amplification conditions were optimized with pure cultures and reactions were carried out to define selectivity, specificity and sensitivity of both primer pairs. The performance of the invA primer pair was better than that of the phoE pair, making the specific detection of Salmonella serovars and strains isolated from different food samples possible. Using the invA primer pair, the combined selective enrichment method with the polymerase chain reaction assay was established and used to detect Salmonella from artificially multi-contaminated food samples. The complete procedure detected as few as three cells of Salmonella (3 c.f.u.) from milk and meat samples.[PUBLICATION ABSTRACT]
ISSN:0959-3993
1573-0972
DOI:10.1023/A:1018514803821