Enzymes catalyzed esterification of N-protected amino acids with secondary alcohols

Secondary alcohols were converted to their corresponding esters with N-Cbz-L-amino acids by Celite-immobilized protease or lipase in organic solvents at pH 7.5. The esterification of 2-butanol and 2-phenylethanol were achieved up to 71% yields. The optimal reaction condition for Aspergillus oryzae p...

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Bibliographic Details
Published in:Biotechnology letters 1997-09, Vol.19 (9), p.857-859
Main Authors: Shih, I.L, Chiu, L.C, Lai, C.T, Liaw, W.C, Tai, D.F
Format: Article
Language:English
Subjects:
Amino acids
Aspergillus oryzae
Bioconversions. Hemisynthesis
Biological and medical sciences
Biotechnology
Enzymes
Esters
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Organic solvents
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Summary:Secondary alcohols were converted to their corresponding esters with N-Cbz-L-amino acids by Celite-immobilized protease or lipase in organic solvents at pH 7.5. The esterification of 2-butanol and 2-phenylethanol were achieved up to 71% yields. The optimal reaction condition for Aspergillus oryzae protease (AOP) catalyzed synthesis of N-Cbz-L-aspartyl-sec-butyl ester (97.6 de) was at pH 7.5 and a duration of 96 hours.
ISSN:0141-5492
1573-6776
DOI:10.1023/A:1018329402408