1,2-propanediol-induced premature centromere separation in mouse oocytes and aneuploidy in one-cell zygotes

Aneuploidy in germ cells results in reproductive failure and mental and physical disorders in humans. Unfortunately, little is known about the causes and mechanisms of aneuploidy induction. The objective of this study was to test the hypothesis that propylene glycol (1,2-propanediol; PG) induces cyt...

Full description

Saved in:
Bibliographic Details
Published in:Biology of reproduction 1997-07, Vol.57 (1), p.92-98
Main Authors: MAILHES, J. B, YOUNG, D, LONDON, S. N
Format: Article
Language:English
Subjects:
Aneuploidy
Animals
Biological and medical sciences
Birth control
Centromere - drug effects
Cleavage Stage, Ovum
Drug toxicity and drugs side effects treatment
Female
Gynecology. Andrology. Obstetrics
Humans
Male
Medical sciences
Mice
Mice, Inbred ICR
Miscellaneous (drug allergy, mutagens, teratogens...)
Models, Biological
Oocytes - drug effects
Oocytes - ultrastructure
Pharmacology. Drug treatments
Pregnancy
Propylene Glycol
Propylene Glycols - toxicity
Sterility. Assisted procreation
Zygote - drug effects
Zygote - ultrastructure
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Aneuploidy in germ cells results in reproductive failure and mental and physical disorders in humans. Unfortunately, little is known about the causes and mechanisms of aneuploidy induction. The objective of this study was to test the hypothesis that propylene glycol (1,2-propanediol; PG) induces cytogenetic aberrations in mouse metaphase II (MII) oocytes that predispose zygotes to aneuploidy. Female ICR mice received 7.5 IU eCG and 5.0 IU hCG 48 h later. PG doses of 1300, 2600, and 5200 mg/kg body weight were given 3 h post-hCG; controls received the solvent deionized water. Ovulated oocytes were collected 16 h after administration of PG and processed for cytogenetic analysis. For the one-cell zygote cytogenetic study, females were given PG and paired (1:1) with ICR males for 16 h. Females that mated were given 2 x 10(-3) M colchicine 22 h post-PG, and zygotes were collected 18 h later. PG significantly (p < 0.05) increased both the proportion of MII oocytes with premature centromere separation (PCS) and the proportion of aneuploid one-cell zygotes. These results support the hypothesis that PG-induced PCS in MII oocytes predisposes zygotes to aneuploidy.
ISSN:0006-3363
1529-7268
DOI:10.1095/biolreprod57.1.92