Process monitoring of acetic acid in Escherichia coli cultivation using electrochemical detection in a flow injection system

A flow-injection system for determination of acetic acid in Escherichia coli cultivations with electrochemical detection based on immobilized acetate kinase (AK), pyruvate kinase (PK) and lactate dehydrogenase (LDH) was developed to cope with the problems related to measurements under process condit...

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Bibliographic Details
Published in:Biotechnology techniques 1997-09, Vol.11 (9), p.683-687
Main Authors: TANG, X.-J, TOCAJ, A, HOLST, O, JOHANSSON, G
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Microbial engineering. Fermentation and microbial culture technology
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Summary:A flow-injection system for determination of acetic acid in Escherichia coli cultivations with electrochemical detection based on immobilized acetate kinase (AK), pyruvate kinase (PK) and lactate dehydrogenase (LDH) was developed to cope with the problems related to measurements under process conditions such as interferences from pyruvate, drift of electrode baseline and making the cultivation medium and reagents compatible. The results obtained by flow injection analysis were compared with those obtained with an enzymatic test kit.
ISSN:0951-208X
1573-6784
DOI:10.1023/A:1018467612171