Antiulcer activity of the ethanolic extract and ethyl acetate fraction of the leaves of Markhamia tomentosa in rats

• Published in: Journal of ethnopharmacology 2014-11, Vol.157, p.1-6
• Main Authors: Sofidiya, Margaret O., Agunbiade, Foluso O., Koorbanally, Neil A., Sowemimo, Abimbola, Soesan, Dapo, Familusi, Titi
• Format: Article
• Language: English
• Subjects: Acetates - chemistry; Animals; Anti-Ulcer Agents - administration & dosage; Anti-Ulcer Agents - pharmacology; Antiulcer; Bignoniaceae; Bignoniaceae - chemistry; Chromatography, Liquid; Disease Models, Animal; Dose-Response Relationship, Drug; Ethanol - chemistry; Indomethacin - toxicity; LC–MS analysis; Male; Markhamia tomentosa; Mass Spectrometry; Medicine, Traditional; Plant Extracts - administration & dosage; Plant Extracts - pharmacology; Plant Leaves; Rats; Rats, Wistar; Solvents; Stomach Ulcer - drug therapy; Stomach Ulcer - pathology
• ISSN: 0378-8741; 1872-7573
• DOI: 10.1016/j.jep.2014.09.012

The leaves of Markhamia tomentosa (Benth.) K. Schum. Ex Engl. (Bignoniaceae) are used traditionally in the treatment of skin afflictions, sores, ulcers and inflammation. The aim of the study was to investigate the antiulcer activity of the crude ethanolic extract from the leaves of Markhamia tomentosa, determine the active fraction(s) of the extract and identify the chemical constituents in the active fraction by LC–MS. The antiulcer activity of the crude extract (50, 100 and 150mg/kg, p.o.) was evaluated in ethanol and indomethacin-induced models while the solvent fractions (150mg/kg) were screened using ethanol-induced gastric lesions in rats. Furthermore, anti-ulcer activity of the active fraction (50, 100 and 150mg/kg, p.o.) was performed using indomethacin and pylorus ligation models. Parameters such as gastric volume, pH and acidity were determined in the pylorus ligation model. LC–ESI–MS analysis was used to identify the components in the active fraction. The extract at the dose of 50, 100 and 150mg/kg caused a significant (p