High resolution/accurate mass (HRMS) detection of anatoxin-a in lake water using LDTD–APCI coupled to a Q-Exactive mass spectrometer

A new innovative analytical method combining ultra-fast analysis time with high resolution/accurate mass detection was developed to eliminate the misidentification of anatoxin-a (ANA-a), a cyanobacterial toxin, from the natural amino acid phenylalanine (PHE). This was achieved by using the laser dio...

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Bibliographic Details
Published in:Talanta (Oxford) 2015-01, Vol.132, p.836-844
Main Authors: Roy-Lachapelle, Audrey, Solliec, Morgan, Sinotte, Marc, Deblois, Christian, Sauvé, Sébastien
Format: Article
Language:English
Subjects:
Anabaena - chemistry
Anatoxin-a
Calibration
Cyanobacteria
Harmful Algal Bloom
High resolution mass spectrometry
Lakes
LDTD–APCI
Limit of Detection
Mass Spectrometry - instrumentation
Mass Spectrometry - methods
Observer Variation
Phenylalanine
Phenylalanine - analysis
Reference Standards
Time Factors
Tropanes - analysis
Water Pollutants, Chemical - analysis
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Summary:A new innovative analytical method combining ultra-fast analysis time with high resolution/accurate mass detection was developed to eliminate the misidentification of anatoxin-a (ANA-a), a cyanobacterial toxin, from the natural amino acid phenylalanine (PHE). This was achieved by using the laser diode thermal desorption–atmospheric pressure chemical ionization (LDTD–APCI) coupled to the Q-Exactive, a high resolution/accurate mass spectrometer (HRMS). This novel combination, the LDTD–APCI–HRMS, allowed for an ultra-fast analysis time (0.999). Enhancement of signal to noise ratios relative to a standard triple-quadrupole method was demonstrated with lower detection and quantification limit values of 0.2 and 0.6μg/L using the Q-Exactive. Accuracy and interday/intraday relative standard deviations were below 15%. The new method was applied to 8 different lake water samples with signs of cyanobacterial blooms. This work demonstrates the possibility of using an ultra-fast LDTD–APCI sample introduction system with an HRMS hybrid instrument for quantitative purposes with high selectivity in complex environmental matrices. [Display omitted] •Anatoxin-a is analysed by LDTD–APCI–HRMS in less than 15s/sample.•Phenylalanine interference is eliminated with high resolution detection.•Two acquisition modes are compared; full scan and targeted ion fragmentation.•The method is applied to real bloom samples with limits of detection of 0.2mg/L.
ISSN:0039-9140
1873-3573
DOI:10.1016/j.talanta.2014.10.021