1,8-Cineole ameliorates oxygen-glucose deprivation/reoxygenation-induced ischaemic injury by reducing oxidative stress in rat cortical neuron/glia

• Published in: Journal of pharmacy and pharmacology 2014-12, Vol.66 (12), p.1818-1826
• Main Authors: Ryu, Sangwoo, Park, Hyeon, Seol, Geun Hee, Choi, In-Young
• Format: Article
• Language: English
• Subjects: 1,8‐cineole; 8-cineole; Animals; Calcium - metabolism; Cell Culture Techniques; Cell Death - drug effects; Cells, Cultured; Cerebral Cortex - cytology; Cerebral Cortex - embryology; cerebral ischaemia; Cyclohexanols - pharmacology; Glucose - metabolism; Hypoxia-Ischemia, Brain - metabolism; Hypoxia-Ischemia, Brain - prevention & control; Microscopy, Phase-Contrast; Monoterpenes - pharmacology; Neuroglia - drug effects; Neuroglia - enzymology; Neuroglia - metabolism; Neurons - drug effects; Neurons - enzymology; Neurons - metabolism; Neuroprotective Agents - pharmacology; oxidative stress; Oxidative Stress - drug effects; Oxygen; Oxygen - metabolism; oxygen-glucose deprivation; Rats, Sprague-Dawley; Reactive Oxygen Species - metabolism; Rodents; superoxide dismutase
• ISSN: 0022-3573; 2042-7158
• DOI: 10.1111/jphp.12295

Objectives 1,8‐Cineole, the main monoterpene in many essential oils, has been used as an ingredient in flavourings and medicine. 1,8‐Cineole has been shown to possess pharmacological properties, including anti‐oxidative, anti‐inflammatory and anti‐nociceptive actions. However, to date, no studies have examined the potential of 1,8‐cineole to protect against cerebral ischaemic injury. Methods In this study, we investigated the neuroprotective effects of 1,8‐cineole against cortical neuronal/glial cell injury caused by oxygen‐glucose deprivation/reoxygenation (OGD/R) in an in‐vitro model of ischaemia. Key findings 1,8‐Cineole significantly attenuated OGD/R‐induced cortical cell injury, as well as reduced n‐methyl‐d‐aspartate (NMDA)‐induced cell injury. However, it did not inhibit NMDA‐induced cytosolic calcium overload. Nevertheless, 1,8‐cineole significantly reduced the OGD/R‐ and NMDA‐induced overproduction of reactive oxygen species (ROS). These results indicate that 1,8‐cineole exerts neuroprotection through its anti‐oxidative rather than its anti‐excitotoxic, properties. The decrease in OGD/R‐induced intracellular superoxide in 1,8‐cineole‐treated cortical cells was associated with the upregulation of superoxide dismutase activity. Moreover, 1,8‐cineole showed direct ROS scavenging activity in an assay of oxygen radical absorbance capacity. Conclusion Collectively, these results suggest 1,8‐cineole as a potentially effective neuroprotective and anti‐oxidative candidate for the treatment of patients with ischaemic stroke.