CD56 Antibody: Old-Fashioned or Still Trendy in Endocrine Lung Tumors

Background: In 2010, the World Health Organization published a new classification of the endocrine tumors based on the mitotic rate and index. Concerning lung endocrine tumors, the classification of 2004 remains acceptable and widely approved. We noticed in many publications that the most used antib...

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Bibliographic Details
Published in:Journal of immunoassay & immunochemistry 2015-07, Vol.36 (4), p.414-419
Main Authors: Mlika, Mona, Zendah, Ines, Braham, Emna, El Mezni, Faouzi
Format: Article
Language:English
Subjects:
Antibodies - immunology
CD56 antibody
CD56 Antigen - immunology
Chromogranins - immunology
Female
Humans
immunohistochemistry
lung endocrine tumors
Lung Neoplasms - diagnosis
Lung Neoplasms - immunology
Male
Middle Aged
Synaptophysin - immunology
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Summary:Background: In 2010, the World Health Organization published a new classification of the endocrine tumors based on the mitotic rate and index. Concerning lung endocrine tumors, the classification of 2004 remains acceptable and widely approved. We noticed in many publications that the most used antibodies in these tumors are chromogranin and synaptophysin. This finding let us wonder about the diagnostic utility of the CD56 antibody which is widely used in our department. Material and methods: Sixty-nine endocrine lung cancers were diagnosed over a 12-month period in our Department of Pathology. Immunohistochemical technique using the three antibodies: chromogranin, synaptophysin, and CD56 was performed. The sensitivity of the three antibodies was performed using the ratio: true negative cases/true negative cases + false positive cases. The specificity wasn't performed because the antibodies were used only in endocrine tumors. The comparison of the different percentages of expression of the three antibodies was made by the SPSS software 22.0. Results: The sensitivity of the chromogranin, synpatophysin, and CD56 accounted for 69%, 77%, and 98%, respectively. The mean percentage of immunoreactive cells with CD56 was 70% towards 15% and 20% with chromogranin and synaptophysin antibodies, respectively. The comparison of the percentages of expression showed a significant statistical difference between the expression of CD56 versus synaptophysin and CD56 versus chromogranin with P
ISSN:1532-1819
1532-4230
DOI:10.1080/15321819.2014.952444