Microwave-assisted extraction of polysaccharides from mulberry leaves

In this study, microwave-assisted extraction (MAE) of polysaccharides from mulberry leaves was investigated using response surface methodology (RSM). The effects of three extraction factors on the yield of polysaccharides was examined. The results showed that optimum extraction conditions were deter...

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Bibliographic Details
Published in:International journal of biological macromolecules 2015-01, Vol.72, p.1-5
Main Authors: Thirugnanasambandham, K, Sivakumar, V, Maran, J Prakash
Format: Article
Language:English
Subjects:
Analysis of Variance
Biotechnology - methods
Complex Mixtures
Microwaves
Models, Theoretical
Molecular Weight
Morus - chemistry
Plant Leaves - chemistry
Polysaccharides - chemistry
Polysaccharides - isolation & purification
Spectroscopy, Fourier Transform Infrared
Time Factors
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Summary:In this study, microwave-assisted extraction (MAE) of polysaccharides from mulberry leaves was investigated using response surface methodology (RSM). The effects of three extraction factors on the yield of polysaccharides was examined. The results showed that optimum extraction conditions were determined as follows: weight of the sample of 20 g, microwave power of 170 W, extraction time of 10 min. Under these optimal extraction conditions, polysaccharide yield was found to be 9.41%. Three factors-three level Box-Behnken response surface design (BBD) coupled with RSM was used to model the extraction process. ANOVA was used to examine the statistical significance of the developed model. Extracted polysaccharide was analyzed using Fourier transform infrared (FT-IR) spectroscopy.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2014.07.031