Structural characterization and bioactivities of sulfated polysaccharide from Monostroma oxyspermum

Sulfated polysaccharide was isolated from Monostroma oxyspermum through hot water extraction, anion-exchange and gel permeation column chromatography. The sulfated polysaccharide contained 92% of carbohydrate, 0% of protein, 7.8% of uronic acid, 22% of ash and 33% of moisture respectively. The eleme...

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Published in:International journal of biological macromolecules 2015-01, Vol.72, p.1459-1465
Main Authors: Seedevi, Palaniappan, Moovendhan, Meivelu, Sudharsan, Sadhasivam, Vasanthkumar, Shanmugam, Srinivasan, Alagiri, Vairamani, Shanmugam, Shanmugam, Annaian
Format: Article
Language:English
Subjects:
Anticoagulants - pharmacology
Antioxidants - pharmacology
Benzothiazoles - chemistry
Biphenyl Compounds - chemistry
Chlorophyta - chemistry
Free Radical Scavengers - pharmacology
Humans
Molecular Weight
Monosaccharides - analysis
Oxidation-Reduction
Picrates - chemistry
Polysaccharides - chemistry
Polysaccharides - pharmacology
Proton Magnetic Resonance Spectroscopy
Spectroscopy, Fourier Transform Infrared
Sulfonic Acids - chemistry
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Summary:Sulfated polysaccharide was isolated from Monostroma oxyspermum through hot water extraction, anion-exchange and gel permeation column chromatography. The sulfated polysaccharide contained 92% of carbohydrate, 0% of protein, 7.8% of uronic acid, 22% of ash and 33% of moisture respectively. The elemental composition was analyzed using CHNS/O analyzer. The molecular weight of sulfated polysaccharide determined through PAGE was found to be as 55 kDa. Monosaccharides analysis revealed that sulfated polysaccharide was composed of rhamnose, fructose, galactose, xylose, and glucose. The structural features of sulfated polysaccharide were analyzed by NMR spectroscopy. Further the sulfated polysaccharide showed total antioxidant and DPPH free radical scavenging activity were as 66.29% at 250 μg/ml and 66.83% at 160 μg/ml respectively. The sulfated polysaccharide also showed ABTS scavenging ability and reducing power were as 83.88% at 125 μg/ml and 15.81% at 400 μg/ml respectively. The anticoagulant activity was determined for human plasma with respect to Activated Partial Thromboplastin Time (APTT) and Prothrombin Time (PT) was 20.09 IU and 1.79 IU at 25 μg/ml respectively. These results indicated that the sulfated polysaccharide from M. oxyspermum had potent antioxidant and anticoagulant activities.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2014.09.062