The growth of yeast thiolase crystals using a polyacrylamide gel as dialysis membrane

3-KetoacylCoA thiolase from yeast Saccharomyces cerevisiae was crystallized at low ionic strength using a micro-dialysis crystallization method. The rate of dialysis was important for obtaining well diffracting crystals. Therefore a polycrylamide gel plug was used as the dialysis membrane. The optim...

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Bibliographic Details
Published in:Journal of crystal growth 1992-08, Vol.122 (1), p.194-198
Main Authors: Zeelen, J.Ph, Wierenga, R.K.
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Enzymes and enzyme inhibitors
Fundamental and applied biological sciences. Psychology
Hydrolases
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Summary:3-KetoacylCoA thiolase from yeast Saccharomyces cerevisiae was crystallized at low ionic strength using a micro-dialysis crystallization method. The rate of dialysis was important for obtaining well diffracting crystals. Therefore a polycrylamide gel plug was used as the dialysis membrane. The optimal rate of dialysis was found by varying the height of the polyacrylamide plug.
ISSN:0022-0248
1873-5002
DOI:10.1016/0022-0248(92)90244-D