Comparison of preparative reversed phase liquid chromatography and countercurrent chromatography for the kilogram scale purification of crude spinetoram insecticide

Reversed phase HPLC (RP-HPLC) and high performance countercurrent chromatography (HPCCC) were compared for the pilot scale purification of two semi-synthetic spinosyns, spinetoram-J and spinetoram-L, the major components of the commercial insecticide spinetoram. Two, independently performed, 1kg, pu...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Chromatography A 2011-09, Vol.1218 (36), p.6122-6127
Main Authors: DeAmicis, Carl, Edwards, Neil A., Giles, Michael B., Harris, Guy H., Hewitson, Peter, Janaway, Lee, Ignatova, Svetlana
Format: Article
Language:English
Subjects:
Analytical chemistry
Biological and medical sciences
Chemical control
Chemistry
Chromatographic methods and physical methods associated with chromatography
Control
countercurrent chromatography
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
HPCCC
HPLC
insecticides
Other chromatographic methods
Phytopathology. Animal pests. Plant and forest protection
Pilot scale
Protozoa. Invertebrates
reversed-phase high performance liquid chromatography
reversed-phase liquid chromatography
solvents
Spinetoram
Spinosyns
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Reversed phase HPLC (RP-HPLC) and high performance countercurrent chromatography (HPCCC) were compared for the pilot scale purification of two semi-synthetic spinosyns, spinetoram-J and spinetoram-L, the major components of the commercial insecticide spinetoram. Two, independently performed, 1kg, purification campaigns were compared. Each method resulted in the isolation of both components at a purity of >97% and yields for spinetoram-J and spinetoram-L of >93% and ≥63% of theoretical, respectively. The HPCCC process produced a 2-fold higher throughput and consumed approximately 70% less solvent than preparative scale RP-HPLC, the volume of product containing fractions from HPCCC amounted to 7% of that produced by HPLC and so required much less post-run processing.
ISSN:0021-9673
DOI:10.1016/j.chroma.2011.06.073