Differentially expressed drug-resistant HIV subpopulations identified by surface marker immunocapture

Persisting, including hidden, drug-resistant HIV can decrease the effectiveness of anti-retroviral therapy (ART) and pose a risk for transmitted resistance. Host cell surface proteins incorporated in the envelope of HIV virions can reveal their cellular origin. We developed an immunomagnetic capture...

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Bibliographic Details
Published in:Antiviral therapy 2014-01, Vol.19, p.A52-A52
Main Authors: Malik, S, Morris, L, Yang, C, Zeh, C, Kiarie, J, Stringer, J SA, Weidle, P J, Johnson, J A
Format: Article
Language:English
Subjects:
Human immunodeficiency virus
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Summary:Persisting, including hidden, drug-resistant HIV can decrease the effectiveness of anti-retroviral therapy (ART) and pose a risk for transmitted resistance. Host cell surface proteins incorporated in the envelope of HIV virions can reveal their cellular origin. We developed an immunomagnetic capture assay to identify cellular sources of expressed drug-resistant virions. The HIV immunomagnetic capture algorithm identified distinct drug-resistant variants expressed from diverse cellular reservoirs, with most expressed mutations undetected by bulk sequencing originating from virion with markers for classical Mc/macrophages and homing Mc. Further examination of these subpopulations may improve our understanding of resistant variant persistence, transmission, and responses to ART.
ISSN:1359-6535