γ-Oryzanol protects against acute cadmium-induced oxidative damage in mice testes

• Published in: Food and chemical toxicology 2013-05, Vol.55, p.526-532
• Main Authors: Spiazzi, Cristiano C., Manfredini, Vanusa, Barcellos da Silva, Fabiana E., Flores, Érico M.M., Izaguirry, Aryele P., Vargas, Laura M., Soares, Melina B., Santos, Francielli W.
• Format: Article
• Language: English
• Subjects: Animals; Antioxidant; Antioxidants - metabolism; ascorbic acid; Biological and medical sciences; Cadmium; Cadmium - toxicity; canola oil; catalase; Chemical and industrial products toxicology. Toxic occupational diseases; Comet Assay; DNA Damage; Enzymes - metabolism; glutathione peroxidase; glutathione transferase; heavy metals; lipid peroxidation; Male; Medical sciences; medicinal properties; Metals and various inorganic compounds; Mice; oryzanol; Oxidative stress; Oxidative Stress - drug effects; Phenylpropionates - pharmacology; protective effect; superoxide dismutase; Testes; Testis - drug effects; Testis - enzymology; Testis - metabolism; therapeutics; thiobarbituric acid-reactive substances; thiols; toxicity; Toxicology; γ-Oryzanol
• ISSN: 0278-6915; 1873-6351
• DOI: 10.1016/j.fct.2013.01.048

[Display omitted] ► Cadmium caused oxidative damage in mice testis acutely exposed to this metal. ► Cadmium exposure affected cell lipids, proteins, DNA, enzymatic and non-enzymatic antioxidants in mice testis. ► γ-Oryzanol therapy restored lipid peroxidation and SOD and GST activities to the control levels. ► Therapy with γ-oryzanol improved partially testicular δ-ALA-D activity inhibited by acute cadmium exposure. ► Cadmium accumulated in testis after acute exposure and the therapy with γ-oryzanol reduced this accumulation in 23%. Cadmium is a non-essential heavy metal that is present at low levels mainly in food and water and also in cigar smoke. The present study evaluated the testicular damage caused by acute cadmium exposure and verified the protective role of γ-oryzanol (ORY). Mice were administrated with a single dose of 2.5mg/kg of CdCl2, and then treated with ORY (50mM in canola oil, 5mL/kg). Testes were removed after 24h and tested for lipid peroxidation (TBARS), protein carbonylation, DNA breakage, ascorbic acid, cadmium and non-proteic thiols contents, and for the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione S-transferase (GST) and δ-aminolevulic acid dehydratase (δ-ALA-D). Cadmium presented a significant alteration in all parameters, except GPx and CAT activities. Therapy reduced in a slight degree cadmium concentration in testes (around 23%). ORY restored SOD and GST activities as well as TBARS production to the control levels. Furthermore, ORY partially recovered δ-ALA-D activity inhibited by cadmium. This study provides the first evidence on the therapeutic properties of ORY in protecting against cadmium-induced testicular toxicity.