The key involvement of estrogen receptor β and G-protein-coupled receptor 30 in the neuroprotective action of daidzein

• Published in: Neuroscience 2013-05, Vol.238, p.345-360
• Main Authors: Kajta, M, Rzemieniec, J, Litwa, E, Lason, W, Lenartowicz, M, Krzeptowski, W, Wojtowicz, A.K
• Format: Article
• Language: English
• Subjects: Animals; apoptosis; Apoptosis - drug effects; Apoptosis - physiology; Benzodioxoles - pharmacology; Biological and medical sciences; Caspase 3 - metabolism; Cells, Cultured; Cerebellum - cytology; Cerebellum - drug effects; Cerebellum - metabolism; Estradiol - analogs & derivatives; Estradiol - pharmacology; Estrogen Receptor beta - antagonists & inhibitors; Estrogen Receptor beta - metabolism; Fundamental and applied biological sciences. Psychology; Glutamic Acid - pharmacology; Hippocampus - cytology; Hippocampus - drug effects; Hippocampus - metabolism; Isoflavones - pharmacology; membrane estrogen receptor; Membrane Potential, Mitochondrial - drug effects; Membrane Potential, Mitochondrial - physiology; Mice; Neocortex - cytology; Neocortex - drug effects; Neocortex - metabolism; Neurology; Neurons - cytology; Neurons - drug effects; Neurons - metabolism; Neuroprotective Agents - pharmacology; neurotoxicity; Phosphorylation - drug effects; phytoestrogen; Piperidines - pharmacology; primary neuronal cell cultures; Pyrazoles - pharmacology; Quinolines - pharmacology; Receptors, Estrogen; Receptors, G-Protein-Coupled - antagonists & inhibitors; Receptors, G-Protein-Coupled - metabolism; Signal Transduction - drug effects; Signal Transduction - physiology; Vertebrates: nervous system and sense organs
• ISSN: 0306-4522; 1873-7544
• DOI: 10.1016/j.neuroscience.2013.02.005

Highlights ► This study has demonstrated a key role of ERβ and GPR30 in the neuroprotective action of daidzein. ► Daidzein inhibited glutamate-induced apoptosis and neurotoxicity. ► Daidzein inhibited glutamate-stimulated protein expression of ERβ, without a significant change of GPR30. ► Involvement of ERβ and GPR30 in daidzein neuroprotection was verified with selective ligands and siRNAs. ► Biochemical data were supported by ERβ- and GPR30-specific immunofluorescence and confocal microscopy.