Differential Cytokine Expression and Regulatory Cells in Patients with Primary and Secondary Sjögren's Syndrome

Sjögren's syndrome (SS) is an autoimmune disease characterized by lymphocytic infiltration of the salivary and lacrimal glands. The aim of the study was to characterize and compare the presence of diverse cytokines and regulatory T and B cells in lip minor salivary gland (MSG) biopsies from pat...

Full description

Saved in:
Bibliographic Details
Published in:Scandinavian journal of immunology 2014-12, Vol.80 (6), p.432-440
Main Authors: Furuzawa‐Carballeda, J., Sánchez‐Guerrero, J., Betanzos, J. L., Enriquez, A. B., Avila‐Casado, C., Llorente, L., Hernández‐Molina, G.
Format: Article
Language:English
Subjects:
Adult
Antigens, Surface - metabolism
B-Lymphocytes, Regulatory - immunology
B-Lymphocytes, Regulatory - metabolism
Biomarkers - metabolism
Biopsy
Cytokines - metabolism
Humans
Immunohistochemistry
Middle Aged
Salivary Glands, Minor - immunology
Salivary Glands, Minor - metabolism
Salivary Glands, Minor - pathology
Sjogren's Syndrome - immunology
Sjogren's Syndrome - metabolism
Sjogren's Syndrome - pathology
T-Lymphocytes, Regulatory - immunology
T-Lymphocytes, Regulatory - metabolism
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Sjögren's syndrome (SS) is an autoimmune disease characterized by lymphocytic infiltration of the salivary and lacrimal glands. The aim of the study was to characterize and compare the presence of diverse cytokines and regulatory T and B cells in lip minor salivary gland (MSG) biopsies from patients with primary Sjögren's syndrome (pSS), secondary SS (sSS), and patients with connective tissue disease (CTD) without (w/o) SS. We included samples of MSG from 15 pSS, 24 sSS (six scleroderma, nine rheumatoid arthritis and nine lupus patients) and 15 patients with CTD w/o SS. Tissues were examined by an indirect immunoperoxidase technique (goat polyclonal anti‐human IL‐19, goat polyclonal anti‐human IL‐22 or mouse monoclonal anti‐human IL‐24). To determine the subpopulation of CD4+/IL‐17A+‐, CD4+/IL‐4+‐, CD4+/IFN‐ɣ+‐expressing T cells, CD25+/Foxp3+ Treg cells and CD20+/IL‐10+‐producing B cell subset, a double‐staining procedure was performed. We estimated the mean percentage of positively staining cells in two fields per sample. CD4+/IFN‐ɣ+, CD4+/IL‐4+ and IL‐22+ cell percentages were elevated in both SS varieties; however, the cells were more prevalent in pSS. Patients with pSS had a high number of CD4+/IL‐17A+ and IL‐19+ T cells and a lower percentage of IL‐24+ cells (P 
ISSN:0300-9475
1365-3083
DOI:10.1111/sji.12224