Distribution of 13 virulence genes among clinical and environmental Aeromonas spp. in Western Australia

We evaluated the pathogenic potential of 98 clinical and 31 environmental Aeromonas isolates by detecting the presence of 13 virulence genes using a polymerase chain reaction (PCR)-based method. The majority (96 %) of the strains contained at least one of the virulence genes. The overall distributio...

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Bibliographic Details
Published in:European journal of clinical microbiology & infectious diseases 2014-11, Vol.33 (11), p.1889-1895
Main Authors: Aravena-Román, M., Inglis, T. J. J., Riley, T. V., Chang, B. J.
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aeromonas
Aeromonas - classification
Aeromonas - genetics
Aeromonas - isolation & purification
Aeromonas veronii
Aged
Aged, 80 and over
Biological and medical sciences
Biomedical and Life Sciences
Biomedicine
Child
Child, Preschool
DNA, Bacterial - genetics
Environmental Microbiology
Female
Genes, Bacterial
Genotype
Gram-Negative Bacterial Infections - microbiology
Humans
Infant
Infectious diseases
Internal Medicine
Laboratory animals
Male
Medical Microbiology
Medical sciences
Middle Aged
Polymerase Chain Reaction
Toxins
Virulence
Virulence Factors - genetics
Western Australia
Young Adult
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Summary:We evaluated the pathogenic potential of 98 clinical and 31 environmental Aeromonas isolates by detecting the presence of 13 virulence genes using a polymerase chain reaction (PCR)-based method. The majority (96 %) of the strains contained at least one of the virulence genes. The overall distribution was aerA / haem (77 %), alt (53 %), lafA (51 %), ast (39 %), flaA (32 %), aspA (29 %), vasH (26 %), ascV (16 %) and aexT (13 %). No amplification products were detected for the genes encoding a bundle-forming pilus ( BfpA and BfpG ) or a Shiga-like toxin ( stx - 1 and stx - 2 ). Five or more virulence genes were detected in 42 % of environmental and 24 % of clinical isolates. Among the major species, 48 % of A. hydrophila and 42 % of A. dhakensis isolates harboured five or more virulence genes compared with 19 % in A. veronii bv. sobria and none in A. caviae isolates. Our results suggest that, in Western Australia, strains of A. dhakensis and A. hydrophila are potentially more virulent than those of A. veronii bv. sobria and A. caviae , although the pathogenic potential of Aeromonas spp. is probably strain- rather than species-dependent.
ISSN:0934-9723
1435-4373
DOI:10.1007/s10096-014-2157-0