Probing bacterial-fungal interactions at the single cell level

Interactions between fungi and prokaryotes are abundant in many ecological systems. A wide variety of biomolecules regulate such interactions and many of them have found medicinal or biotechnological applications. However, studying a fungal-bacterial system at a cellular level is technically challen...

Full description

Saved in:
Bibliographic Details
Published in:Integrative biology (Cambridge) 2014-10, Vol.6 (10), p.935-945
Main Authors: Stanley, Claire E, Stöckli, Martina, van Swaay, Dirk, Sabotič, Jerica, Kallio, Pauli T, Künzler, Markus, deMello, Andrew J, Aebi, Markus
Format: Article
Language:English
Subjects:
Bacillus subtilis
Bacillus subtilis - growth & development
Bacillus subtilis - ultrastructure
Basidiomycetes
Basidiomycota - growth & development
Basidiomycota - ultrastructure
Hyphae - growth & development
Hyphae - ultrastructure
Microfluidics - methods
Microscopy, Fluorescence
Time-Lapse Imaging
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Interactions between fungi and prokaryotes are abundant in many ecological systems. A wide variety of biomolecules regulate such interactions and many of them have found medicinal or biotechnological applications. However, studying a fungal-bacterial system at a cellular level is technically challenging. New microfluidic devices provided a platform for microscopic studies and for long-term, time-lapse experiments. Application of these novel tools revealed insights into the dynamic interactions between the basidiomycete Coprinopsis cinerea and the bacterium Bacillus subtilis. Direct contact was mediated by polar attachment of bacteria to only a subset of fungal hyphae suggesting a differential competence of fungal hyphae and thus differentiation of hyphae within a mycelium. The fungicidal activity of B. subtilis was monitored at a cellular level and showed a novel mode of action on fungal hyphae.
ISSN:1757-9694
1757-9708
DOI:10.1039/c4ib00154k