Poplar GATA transcription factor PdGNC is capable of regulating chloroplast ultrastructure, photosynthesis, and vegetative growth in Arabidopsis under varying nitrogen levels

Photosynthesis and nitrogen availability are crucial to the accumulation of biomass. The GATA transcription factor family plays important roles in chloroplast development and nitrogen metabolism. Here, we cloned, for the first time, the GATA transcription factor PdGNC from the fast-growing poplar cl...

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Bibliographic Details
Published in:Plant cell, tissue and organ culture tissue and organ culture, 2014-11, Vol.119 (2), p.313-327
Main Authors: An, Yi, Han, Xiao, Tang, Sha, Xia, Xinli, Yin, Weilun
Format: Article
Language:English
Subjects:
Arabidopsis
Biomedical and Life Sciences
Cell size
Chlorophyll
Chloroplasts
Fluorescence
Growth rate
Leaves
Levels
Life Sciences
Nitrates
Nitrogen
Nitrogen metabolism
Original Paper
Parameter modification
Photosynthesis
Plant Genetics and Genomics
Plant growth
Plant Pathology
Plant Physiology
Plant Sciences
Poplar
Starch
Thylakoids
Transcription factors
Transgenic plants
Ultrastructure
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Summary:Photosynthesis and nitrogen availability are crucial to the accumulation of biomass. The GATA transcription factor family plays important roles in chloroplast development and nitrogen metabolism. Here, we cloned, for the first time, the GATA transcription factor PdGNC from the fast-growing poplar clone NE-19. The overexpression results from Arabidopsis under high nitrate, sufficient nitrate, and low nitrate (LN) support that PdGNC increased the chloroplast number and size per cell in leaf and stem, improved the chlorophyll level by 26.12 % and exhibited the highest starch content in LN. Overexpression of PdGNC also had pronounced effects on chloroplast ultrastructure by increasing the number of grana and thylakoids. The photosynthetic rate in transgenic LN lines was 42.17 % higher than in the wild type through modification of the chlorophyll fluorescence parameters F v /F 0 , F v /F m , qP, NPQ, and Φ PSII . Morphologically, PdGNC promoted longer primary roots and larger leaf areas, and exhibited a higher relative growth rate in LN. Altogether, PdGNC improved photosynthetic capacity and plant growth under low nitrate levels; thus, it could potentially be used in transgenic breeding to improve nitrate utilization and plant growth rates under limited nitrogen conditions.
ISSN:0167-6857
1573-5044
DOI:10.1007/s11240-014-0536-y