Efficacy of a food plant-based oral cholera toxin B subunit vaccine

Transgenic potatoes were engineered to synthesize a cholera toxin B subunit (CTB) pentamer with affinity for G M1 -ganglioside. Both serum and intestinal CTB-specific antibodies were induced in orally immunized mice. Mucosal antibody titers declined gradually after the last immunization but were res...

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Bibliographic Details
Published in:Nature biotechnology 1998-03, Vol.16 (3), p.292-297
Main Authors: Arakawa, T, Chong, D.K.X, Langridge, W.H.R
Format: Article
Language:English
Subjects:
Agriculture
Animals
Antibodies - blood
BACTERIAL TOXINS
Bacteriology
Bioinformatics
Biological and medical sciences
Biomedical and Life Sciences
Biomedical Engineering/Biotechnology
Biomedicine
Biotechnology
Cercopithecus aethiops
Cholera - complications
Cholera - immunology
Cholera Toxin - genetics
Cholera Toxin - immunology
Diarrhea - complications
Diarrhea - immunology
Diarrhea - therapy
Female
Fundamental and applied biological sciences. Psychology
G(M1) Ganglioside - metabolism
Immune Sera
Life Sciences
Mice
Mice, Inbred Strains
Microbiology
Mucous Membrane - immunology
PLANTAS TRANSGENICAS
PLANTE TRANSGENIQUE
Plants, Genetically Modified - genetics
research-article
SOLANUM TUBEROSUM
Solanum tuberosum - genetics
TOXINAS BACTERIANAS
TOXINE BACTERIENNE
TRANSGENIC PLANTS
VACCINE DEVELOPMENT
Vaccines - administration & dosage
Vaccines - genetics
Vaccines - pharmacology
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies
Vero Cells - immunology
Vero Cells - microbiology
VIBRIO CHOLERAE
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Summary:Transgenic potatoes were engineered to synthesize a cholera toxin B subunit (CTB) pentamer with affinity for G M1 -ganglioside. Both serum and intestinal CTB-specific antibodies were induced in orally immunized mice. Mucosal antibody titers declined gradually after the last immunization but were restored following an oral booster of transgenic potato. The cytopathic effect of cholera holotoxin (CT) on Vero cells was neutralized by serum from mice immunized with transgenic potato tissues. Following intraileal injection with CT, the plant-immunized mice showed up to a 60% reduction in diarrheal fluid accumulation in the small intestine. Protection against CT was based on inhibition of enterotoxin binding to the cell-surface receptor G M1 -ganglioside. These results demonstrate the ability of transgenic food plants to generate protective immunity in mice against a bacterial enterotoxin.
ISSN:1087-0156
1546-1696
DOI:10.1038/nbt0398-292