Reproductive cycle and plasma levels of sex steroids in female Eurasian perch Perca fluviatilis

• Published in: Aquatic living resources (Montrouge) 1998, Vol.11 (2), p.101-110
• Main Authors: Sulistyo, Isdy, Rinchard, Jacques, Fontaine, Pascal, Gardeur, Jean-Noël, Capdeville, Bruno, Kestemont, Patrick
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; cycle de reproduction; Eurasian perch; Freshwater; Fundamental and applied biological sciences. Psychology; Non mammalian vertebrate reproduction; oogenesis; ovogenèse; Perca fluviatilis; reproductive cycle; sexual steroids; stéroïdes sexuels; Vertebrates: reproduction
• ISSN: 0990-7440; 1765-2952
• DOI: 10.1016/S0990-7440(98)80066-1

From April 1995 to April 1996, the annual reproductive cycle of the Eurasian perch Perca fluviatilis was studied at the Fishfarming Lindre Center (Moselle, France). At monthly intervals (at intervals of 10 days during the periovulatory period), 5 females were caught and dissected. From sampled organs, the gonado-, hepato- and viscerosomatic indexes (GSI, HSI, VSI) were calculated, oocyte diameters (OD) and the plasma levels of testosterone (T), 17β-estradiol (E 2), 17,20β-dihydroxy-4-pregnen-3-one (17,20β-P) and protein-phosphorus (PPP) were measured. After the sexual resting period observed from May to August (GSI < 1 %, OD < 200 μm, VSI = 4–6 %), oogenesis began in September when the water temperature decreased from 26.4 to 14.1 °C. The GSI increased progressively until mid March (15 %), then rapidly until spawning (25 %, OD = 850 μm) which occurred in April (14–15 °C). The plasma levels of T, E 2, 17,20β-P and PPP were low during the sexual resting period. E 2 and PPP levels increased significantly at the onset of the oogenesis in September, then the E 2 level raised abruptly in November (3–4 ng · mL −1). In December, the T level increased rapidly to 15–20 ng · mL −1. The testosterone, E 2 and PPP levels remained very high until spawning, indicating the existence of active vitellogenesis. The highest HSI (2.1–2.2 %) recorded in winter confirmed this. During the periovulatory period, a peak of E 2 (4 ng · mL −1) appeared, whereas T level diminished. In this study, 17,20β-P levels remained low (0.2–0.6 ng · mL −1) and relatively constant. No 17,20β-P peak was observed during the periovulatory period. Sampling at 10 day intervals was probably inadequate to specify the hormonal variations related to the final oocyte maturation and the ovulation. Entre avril 1995 et avril 1996, une étude a été conduite au Centre Piscicole Départemental du Domaine de Lindre (Moselle, France) pour étudier le cycle annuel de reproduction de la perche eurasienne Perca fluviatilis. Celle-ci s'appuie sur des captures mensuelles (ou tous les 10 jours en période péri-ovulatoire) de 5 femelles, sur lesquelles le prélèvement d'organes a permis le calcul des indices gonado-, hépato- et viscéro-somatique (IGS, IHS, IVS), la mesure du diamètre ovocytaire (DO) et le dosage des teneurs, dans le plasma, en testosterone (T), 17β-estradiol (E 2), 17,20β-dihydroxy-4-pregnen-3-one (17,20β-P) et phosphore protéique (PPP). Après la phase de repos sexuel observée de mai à août (IGS < 1 %, DO < 200