Production of constitutive, thermostable, hyper active exo-pectinase from Bacillus GK-8

A new alkalophilic Bacillus GK-8 overproduced three thermostable extracellular pectinases. The temperature optima was 60 degree C for all the three enzymes which retained full activity for 2 h. They had pH optima 5.4, 7.0 and 10.4 and were designated as PI, PII and PIII respectively. The half life a...

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Bibliographic Details
Published in:Biotechnology letters 1996-12, Vol.18 (12), p.1435-1438
Main Authors: NIRPJIT SINGH DOSANJH, HOONDAL, G. S
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Enzyme engineering
Fermentation
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Production of selected enzymes
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Summary:A new alkalophilic Bacillus GK-8 overproduced three thermostable extracellular pectinases. The temperature optima was 60 degree C for all the three enzymes which retained full activity for 2 h. They had pH optima 5.4, 7.0 and 10.4 and were designated as PI, PII and PIII respectively. The half life at 80 degree C of PI, PII and PIII was 18 min., 12 min. and 12 min., respectively. The enzyme activity was stimulated by Mg super(2+), Ca super(2+), Zn super(2+), Co super(2+) and Mn super(2+) ions (DBO).
ISSN:0141-5492
1573-6776
DOI:10.1007/bf00129350