Effect of media composition on growth, plasmid stability and ovine interferon-τ production in Saccharomyces cerevisiae

• Published in: Journal of fermentation and bioengineering 1996-01, Vol.82 (6), p.565-569
• Main Authors: Chiruvolu, Vijay, Stratton, Jayne M., Ott, Troy L., Bazer, Fuller W., Meagher, Michael M.
• Format: Article
• Language: English
• Subjects: Antineoplastic drugs. Cytokines; Biological and medical sciences; Biotechnology; fed-batch; fermentation; Fundamental and applied biological sciences. Psychology; Health. Pharmaceutical industry; Industrial applications and implications. Economical aspects; interferon-τ; Methods. Procedures. Technologies; Microbial engineering. Fermentation and microbial culture technology; Production of active biomolecules; recombinant; Saccharomyces cerevisiae
• ISSN: 0922-338X
• DOI: 10.1016/S0922-338X(97)81253-6

Recombinant protein production is often influenced by the stability of plasmid carrying the cloned gene and the final biomass yield. Ovine (sheep) interferon-τ (oIFNτ) has been expressed in Saccharomyces cerevisiae under the regulation of a hybrid alcohol dehydrogenase 2/glyceraldehyde-3-phosphate dehydrogenase promoter. The influence of media composition on growth rate, biomass yield, plasmid stability and oIFNτ were studied. In a selective medium, with leucine and uracil selection, the plasmid stability was good but the yeast failed to grow to high cell densities. In 60- l fermentations, oIFNτ yields of 49 mg/ l were achieved for complex media and about 3.4 mg/ l for casamino acids with uracil selection based on bioassay. A fraction of the protein was insoluble and was solubilized by 6 M guanidine hydrochloride.