The in vivo effect of lipopolysaccharide on Na super(+),K super(+)-ATPase catalytic ( alpha ) subunit isoforms in rat sciatic nerve

The Na super(+), K super(+)-ATPase catalytic ( alpha ) subunit in sciatic nerve of lipopolysaccharide (endotoxin, LPS)-treated rat was investigated. Using Western blot to determine subunit isoform polypeptide levels in rat sciatic nerve, we found a substantial reduction in alpha 1 polypeptide, but n...

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Bibliographic Details
Published in:Neuroscience letters 1997-10, Vol.234 (2-3), p.166-168
Main Authors: Liu, Shing H, Sheu, Tzong J
Format: Article
Language:English
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Summary:The Na super(+), K super(+)-ATPase catalytic ( alpha ) subunit in sciatic nerve of lipopolysaccharide (endotoxin, LPS)-treated rat was investigated. Using Western blot to determine subunit isoform polypeptide levels in rat sciatic nerve, we found a substantial reduction in alpha 1 polypeptide, but not that of alpha 2 and alpha 3 polypeptides, after the administration of LPS. Moreover, when rats were treated with polymyxin B (a LPS neutralizer) and N super(G)-nitro-L-arginine (an inhibitor of nitric oxide (NO) synthase), the effects of LPS were reversed. These results implicate a specific marked deficit in alpha 1 subunit isoform of Na super(+),K super(+)-ATPase in the pathogenesis of neuropathy during endotoxemia, through, at least in part, the L-arginine/NO pathway.
ISSN:0304-3940