Toxicity test of biodegradable polymers by implantation in rabbit cornea

To evaluate whether or not the corneal micropocket implantation is effective for determining the toxicity of polymeric materials, currently used biodegradable polymers such as poly(lactic acid) (PLA), poly(glycolic acid) (PGA), LA‐GA copolymers, and three kinds of poly(2‐cyanoacrylate)s (PCA) were i...

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Bibliographic Details
Published in:Journal of biomedical materials research 1992-11, Vol.26 (11), p.1463-1476
Main Authors: Kobayashi, Hisatoshi, Shiraki, Kaneto, Ikada, Yoshito
Format: Article
Language:English
Subjects:
Animals
Biocompatible Materials - toxicity
Biological and medical sciences
Cornea - drug effects
Cornea - pathology
Cyanoacrylates - toxicity
Delayed-Action Preparations
Diseases of the eye
Female
Fibroblast Growth Factor 2 - toxicity
Lactates - toxicity
Lactic Acid
Medical sciences
Polyesters
Polyglactin 910 - toxicity
Polyglycolic Acid - toxicity
Polymers - toxicity
Rabbits
Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)
Reference Values
Time Factors
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Summary:To evaluate whether or not the corneal micropocket implantation is effective for determining the toxicity of polymeric materials, currently used biodegradable polymers such as poly(lactic acid) (PLA), poly(glycolic acid) (PGA), LA‐GA copolymers, and three kinds of poly(2‐cyanoacrylate)s (PCA) were implanted in a rabbit corneal pouch and the tissue responses were observed macroscopically and microscopically. It was found that PLA induced no vascularization, whereas a residual solvent and ethylene oxide gas remaining in the PLA matrix invoked vascularization. Vascularization clearly took place when PGA was implanted in the cornea, which became opaque, probably because of cellular infiltration. In the case of PCA implantation, severe inflammation as well as vascular invasion occurred in the initial stage. It is likely that these tissue reactions were caused by the leachables from the implanted materials, the extent being dependent on the leaching rate and the toxicity. It was concluded that the corneal micropocket assay is a good means to detect trace amounts of leachables from implanted materials without sacrificing the animals with the implanted materials. © 1992 John Wiley & Sons, Inc.
ISSN:0021-9304
1097-4636
DOI:10.1002/jbm.820261106