A new photoreactivating enzyme that specifically repairs ultraviolet light-induced (6-4)photoproducts

Cyclobutane pyrimidine dimers (CPDs) and pyrimidine (6-4) pyrimidone photoproducts ((6-4)photoproducts) are the two major classes of cytotoxic, mutagenic and carcinogenic DNA photoproducts produced by ultraviolet light irradiation of cells. The phenomenon of photoreactivation, the reduction of the l...

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Bibliographic Details
Published in:Nature (London) 1993-01, Vol.361 (6410), p.371-374
Main Authors: Todo, Takeshi, Takemori, Hiroshi, Ryo, Haruko, lhara, Makoto, Matsunaga, Tsukasa, Nikaido, Osamu, Sato, Kenji, Nomura, Taisei
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Biological and medical sciences
Biology
Bombyx
Cell Line
Deoxyribonucleases, Type II Site-Specific
Deoxyribonucleic acid
DNA
DNA - chemistry
DNA - radiation effects
DNA Damage
DNA Repair
Drosophila melanogaster
Drosophila melanogaster - embryology
Embryo, Nonmammalian - enzymology
Enzymes
Escherichia coli - enzymology
Fundamental and applied biological sciences. Psychology
HeLa Cells
Humans
Male
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Mutagenesis. Repair
Pyrimidine Dimers - metabolism
Salmon
Spermatozoa
Ultraviolet radiation
Ultraviolet Rays
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Summary:Cyclobutane pyrimidine dimers (CPDs) and pyrimidine (6-4) pyrimidone photoproducts ((6-4)photoproducts) are the two major classes of cytotoxic, mutagenic and carcinogenic DNA photoproducts produced by ultraviolet light irradiation of cells. The phenomenon of photoreactivation, the reduction of the lethal and mutagenic effects of ultraviolet radiation by simultaneous or subsequent irradiation with near ultraviolet or visible light, has been identified in several organisms and in some cases the enzymes that catalyse this process have been characterized in sufficient detail. CPDs are the only known substrate for the photoreactivating enzymes so far analysed and enzymatic photoreactivation of (6-4)photoproducts has not yet been reported. We report here that an enzyme that catalyses the light-dependent repair of (6-4)photoproduct exists in Drosophila melanogaster. This is, to our knowledge, the first report of such photoreactivating activity specific for (6-4)photoproducts in any organism.
ISSN:0028-0836
1476-4687
DOI:10.1038/361371a0