An antisense chalcone synthase cDNA leads to novel colour patterns in lisianthus (Eustoma grandiflorum) flowers

Three cultivars of lisianthus (Eustoma grandiflorum (Grise.)) were transformed with a homologous antisense CHS cDNA via Agrobacterium-mediated transformation. Over 50% of the transgenics derived from the purple flowering lines exhibited an altered flower colour pattern ranging from small streaks of...

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Bibliographic Details
Published in:Molecular breeding 1998-01, Vol.4 (1), p.59-66
Main Authors: Deroles, S.C. (New Zealand Inst. for Crop and Food Research Ltd., Levin (New Zealand). Levin Research Centre), Bradley, J.M, Schwinn, K.E, Markham, K.R, Bloor, S, Manson, D.G, Davies, K.M
Format: Article
Language:English
Subjects:
ADN
AGROBACTERIUM
Antisense DNA
Biochemical analysis
CALCONA
CHALCONE
Chalcone synthase
CHALCONES
CHS gene
COLOR
COLOUR
COULEUR
Cultivars
DNA
Enzymatic activity
Enzyme activity
EUSTOMA GRANDIFLORUM
Flavonoids
FLEUR
FLORES
Flowering
FLOWERS
GENE TRANSFER
GENETIC TRANSFORMATION
Homology
Molecular biology
Phenotypes
Plant biology
PLANTAS TRANSGENICAS
PLANTE TRANSGENIQUE
Plants (botany)
Stability analysis
Transcription
TRANSFERENCIA DE GENES
TRANSFERT DE GENE
TRANSFORMACION GENETICA
TRANSFORMATION GENETIQUE
TRANSGENIC PLANTS
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Summary:Three cultivars of lisianthus (Eustoma grandiflorum (Grise.)) were transformed with a homologous antisense CHS cDNA via Agrobacterium-mediated transformation. Over 50% of the transgenics derived from the purple flowering lines exhibited an altered flower colour pattern ranging from small streaks of white on the wild-type purple background through to completely white flowers. A significant portion of the transgenic lines showed unstable phenotypes. Northern and biochemical analysis showed that the altered flower patterns were associated with a loss of CHS gene transcript and a corresponding loss of CHS enzyme activity. In the white flowering line the level of total flavonoids was reduced to ca. 2.0% of the wild-type level. Some of the transgenic plants also exhibited alterations in flower form such as the formation of frilled petal tips and reduced flower opening. Several of the new patterned lines are being evaluated for stability and possible commercial release.
ISSN:1380-3743
1572-9788
DOI:10.1023/A:1009621903402