Fine Structure of Neurally Differentiated iPS Cells Generated from a Multiple Sclerosis (MS) Patient: A Case Study

We compared the characteristics of neural cells derived from induced pluripotent stem (iPS) cells from a patient with multiple sclerosis versus neurally differentiated control iPS cells of a healthy individual. The iPS cells were differentiated toward the oligodendrocyte lineage using a four-step pr...

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Bibliographic Details
Published in:Microscopy and microanalysis 2014-12, Vol.20 (6), p.1869-1875
Main Authors: Herszfeld, Daniella, Payne, Natalie L., Sylvain, Aude, Sun, Guizhi, Bernard, Claude C., Clark, Joan, Sathananthan, Henry
Format: Article
Language:English
Subjects:
Biological Applications
Cell Differentiation
Ethanol
Fibroblasts
Humans
Induced Pluripotent Stem Cells - physiology
Induced Pluripotent Stem Cells - ultrastructure
Microscopy
Microscopy, Electron, Transmission
Monoclonal antibodies
Morphology
Multiple Sclerosis
Neural Stem Cells - physiology
Neural Stem Cells - ultrastructure
Polyclonal antibodies
Stem cells
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Summary:We compared the characteristics of neural cells derived from induced pluripotent stem (iPS) cells from a patient with multiple sclerosis versus neurally differentiated control iPS cells of a healthy individual. The iPS cells were differentiated toward the oligodendrocyte lineage using a four-step protocol established for the differentiation of embryonic stem cells. The resulting cell population was immunostained on day 112 of differentiation for the presence of oligodendrocytes and analyzed by transmission electron microscopy (TEM). Both patient and control samples resembled a mixed population of neural cells rather than oligodendroglia of high purity, including neural stem cell-like cells and possibly oligodendrocytes demonstrable by TEM.
ISSN:1431-9276
1435-8115
DOI:10.1017/S1431927614013312