Differential effects of triacylglycerol positional isomers containing n-3 series highly unsaturated fatty acids on lipid metabolism in C57BL/6J mice

• Published in: The Journal of nutritional biochemistry 2015-01, Vol.26 (1), p.57-63
• Main Authors: Yoshinaga, Kazuaki, Sasaki, Keiichi, Watanabe, Hiroyuki, Nagao, Koji, Inoue, Nao, Shirouchi, Bungo, Yanagita, Teruyoshi, Nagai, Toshiharu, Mizobe, Hoyo, Kojima, Koichi, Beppu, Fumiaki, Gotoh, Naohiro
• Format: Article
• Language: English
• Subjects: Administration, Oral; Animals; C57BL/6J mouse; Cholesterol, HDL - blood; Cholesterol, LDL - blood; Docosahexaenoic acid; Docosahexaenoic Acids - administration & dosage; Docosahexaenoic Acids - blood; Eicosapentaenoic acid; Eicosapentaenoic Acid - administration & dosage; Eicosapentaenoic Acid - blood; Isomerism; Lipid metabolism; Lipid Metabolism - drug effects; Liver - drug effects; Liver - metabolism; Male; Mice; Mice, Inbred C57BL; Palmitic Acid - administration & dosage; Positional isomer; Triacylglycerol; Triglycerides - administration & dosage; Triglycerides - blood
• ISSN: 0955-2863; 1873-4847
• DOI: 10.1016/j.jnutbio.2014.09.004

The present study investigated the effects of binding position of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) to triacylglycerol (TAG) on lipid metabolism in C57BL/6J mice. Mice were treated with pure TAG positional isomers, including 1,2(2,3)-dipalmitoyl-3(1)-eicosapentaenoyl glycerol, 1,3-dipalmitoyl-2-eicosapentaenoyl glycerol, 1,2(2,3)-dipalmitoyl-3(1)-docosahexaenoyl glycerol, and 1,3-dipalmitoyl-2-docosahexaenoyl glycerol. Compared to DHA bound to the α-position of TAG, DHA bound to the β-position more effectively inhibited fatty acid synthetic enzymes and cholesterol-metabolism enzymes and thus reduced TAG and cholesterol concentrations in the serum and liver. EPA bound to the α-position of TAG, but not EPA bound to the β-position of TAG, significantly decreased hepatic cholesterol concentrations. Additionally, EPA bound to the α-position of TAG increased the ratio of PGI2 to TXA2 to a higher degree than EPA bound to the β-position. These results suggested that the binding position of EPA and DHA to TAG affected TAG and cholesterol metabolism as well as eicosanoid production in C57BL/6J mice.