Characterization of an adhesive molecule from Bacillus megaterium ADE-0-1

• Published in: Carbohydrate polymers 2015-03, Vol.117, p.543-548
• Main Authors: Kumar, Santosh, Shah, Avinash K.
• Format: Article
• Language: English
• Subjects: Acrylic plastic; Bacillus megaterium; Bacillus megaterium - chemistry; Bacillus megaterium - metabolism; Exopolysaccharide adhesive for wood; Metals; Molecular Weight; Polysaccharides, Bacterial - biosynthesis; Polysaccharides, Bacterial - chemistry; Polysaccharides, Bacterial - isolation & purification
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2014.10.017

•An exopolysaccharide (EPS) exhibited good adhesive property with various surfaces.•The EPS was of high molecular weight and unique chemical compositions.•This is a first report of adhesive-EPS from Bacillus megaterium. An adhesive exopolysaccharide (EPS), from a biofilm forming marine strain ADE-0-1, identified as Bacillus megaterium using conventional microbiological test and 16S rDNA analysis, contained 75% carbohydrate, 17% uronic acid and 0.00125% pyruvate on dry weight basis as per colorimetric determinations and found anionic in nature by ion exchange chromatography. Paper chromatographic and HPLC analysis of EPS hydrolysate indicated presence of arabinose, glucose, mannose, galacturonic acid and glucuronic acid. Its molecular weight was 0.5×106Da, by gel permeation chromatography. FT-IR spectroscopic analysis of EPS revealed presence of hydroxyl and carboxyl groups particularly. EPS exhibited an adhesive nature and could glue wood, metals and acrylic plastic. Using this EPS adhesive (10% w/v), maximum lap shear strength observed was 6.12MPa at pH 7 and 50°C (curing temperature) for wood to wood specimen as compared to 6.54MPa obtained with fevicol (48 to 50% w/v).