Alkali-treated konjac glucomannan film as a novel wound dressing

•Ca(OH)2-KGM films exhibited more favorable properties than KOH–KGM films to be wound dressings.•The Ca(OH)2-KGM films exhibited a suitable WVTR for use as wound dressings.•The Ca(OH)2-KGM film is compatible with L929 cells, HaCaT cells and blood.•The Ca(OH)2-KGM film accelerates wound contraction a...

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Bibliographic Details
Published in:Carbohydrate polymers 2015-03, Vol.117, p.778-787
Main Authors: Huang, Yi-Cheng, Chu, Hao-Wen, Huang, Chih-Ching, Wu, Wen-Ching, Tsai, Jenn-Shou
Format: Article
Language:English
Subjects:
Alkali
Alkalies - chemistry
Animals
Bandages
Biocompatible Materials - chemistry
Biocompatible Materials - pharmacology
Calcium Hydroxide - chemistry
Cell Line
Cell Proliferation - drug effects
Fibroblasts - cytology
Fibroblasts - drug effects
Humans
Hydroxides - chemistry
Konjac glucomannan
Mannans - chemistry
Mannans - pharmacology
Platelet Activation - drug effects
Potassium Compounds - chemistry
Rats
Wound dressing
Wound Healing
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Summary:•Ca(OH)2-KGM films exhibited more favorable properties than KOH–KGM films to be wound dressings.•The Ca(OH)2-KGM films exhibited a suitable WVTR for use as wound dressings.•The Ca(OH)2-KGM film is compatible with L929 cells, HaCaT cells and blood.•The Ca(OH)2-KGM film accelerates wound contraction and reepithelialization processes of wounds.•The alkali-treated KGM films can potentially be used as wound dressings. To investigate the potential medical application of konjac glucomannan (KGM), we treated KGM film with potassium hydroxide (KOH) or calcium hydroxide (Ca(OH)2), and evaluated its use as a wound dressing. The Ca(OH)2-treated KGM (Ca(OH)2-KGM) film exhibited more favorable properties of swelling, tensile strength, and elongation compared with the KOH-treated KGM (KOH–KGM) film, and also had a suitable water vapor transmission rate. Results from in vitro 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay further indicated the biocompatibility of the Ca(OH)2-KGM film with L929 fibroblast cells and HaCaT keratinocyte cells. The Ca(OH)2-KGM film inhibited the absorption and activation of platelets, and effectively promoted wound contractility in vivo, particularly at an early healing stage. Histological examination revealed considerably collagen secretion and advanced development of granulation tissue and epithelial coverage by Days 7 and 14 postsurgery in wounds treated with Ca(OH)2-KGM film. Our study results indicate the potential use of alkali-treated KGM film as a novel wound dressing.
ISSN:0144-8617
1879-1344
DOI:10.1016/j.carbpol.2014.10.047