Rapid identification of Aerococcus viridans using the polymerase chain reaction and an oligonucleotide probe

A polymerase chain reaction/oligonucleotide probe method was developed for the specific identification of the Gram‐positive bacterium Aerococcus viridans. Primers for the enzymatic amplification reaction were designed from specific sequences within the 16S rRNA. The method was also highly sensitive...

Full description

Saved in:
Bibliographic Details
Published in:FEMS microbiology letters 1992-08, Vol.95 (1), p.63-69
Main Authors: Grant, Kathleen A., Dickinson, Joanne H., Collins, Matthew D., Kroll, Rohan G.
Format: Article
Language:English
Subjects:
Aerococcus viridans
Bacteriological methods and techniques used in bacteriology
Bacteriology
Base Sequence
Biological and medical sciences
Blotting, Southern
DNA, Bacterial - genetics
Fundamental and applied biological sciences. Psychology
Identification
Microbiology
Molecular Sequence Data
Oligonucleotide probe
Oligonucleotide Probes
Polymerase Chain Reaction
Sensitivity and Specificity
Staphylococcus - genetics
Streptococcaceae - classification
Streptococcaceae - genetics
Streptococcaceae - isolation & purification
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A polymerase chain reaction/oligonucleotide probe method was developed for the specific identification of the Gram‐positive bacterium Aerococcus viridans. Primers for the enzymatic amplification reaction were designed from specific sequences within the 16S rRNA. The method was also highly sensitive and 10 cfu of A. viridans could be detected in 5 h although the reliability of detection was poor in mixed cultures with Escherichia coli.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.1992.tb05343.x