Sequences of Saccharomyces cerevisiae 2 μm DNA improving plasmid partitioning in Hansenula polymorpha

Insertion of the HindIII‐PstI fragment of Saccharomyces cerevisiae 2 μm DNA into the Hansenula polymorpha replicative plasmids decreases plasmid copy number and ensures their distribution to daughter cells at both mitotic and meiotic cell divisions. This suggests that the stabilization effect is cau...

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Bibliographic Details
Published in:Yeast (Chichester, England) England), 1998-01, Vol.14 (1), p.1-9
Main Authors: Bogdanova, Aliona I., Kustikova, Olga S., Agaphonov, Michael O., Ter‐Avanesyan, Michael D.
Format: Article
Language:English
Subjects:
2 μm DNA
Hansenula polymorpha
nuclear segregation
plasmid partitioning
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Summary:Insertion of the HindIII‐PstI fragment of Saccharomyces cerevisiae 2 μm DNA into the Hansenula polymorpha replicative plasmids decreases plasmid copy number and ensures their distribution to daughter cells at both mitotic and meiotic cell divisions. This suggests that the stabilization effect is caused by the improvement of plasmid partitioning. Deletion analysis revealed that the region of 2 μm DNA sequence responsible for the increase of mitotic stability of H. polymorpha plasmids involves the 2 μm STB locus and adjoining region. Further analysis demonstrated that the stabilization effect may depend on the number of 24–28 bp imperfect repeats which were found in several copies in the STB locus and adjoining region. © 1998 John Wiley & Sons, Ltd.
ISSN:0749-503X
1097-0061
DOI:10.1002/(SICI)1097-0061(19980115)14:1<1::AID-YEA195>3.0.CO;2-D