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Developmental competence of frozen-thawed yak (Bos grunniens) oocytes followed by in vitro maturation and fertilization
In the present study, we examined the ability of immature germinal vesicle (GV) and subjected to in vitro matured (MII) yak oocytes to survive after cryopreservation as well as their subsequent development following in vitro maturation and fertilization. Both GV and MII oocytes were cryopreserved by...
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| Published in: | Cryobiology 2014-02, Vol.68 (1), p.152-154 |
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| Main Authors: | , , , , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c467t-927ffd5fabb985ecc11aed740082a84beef097db8baec5b480edcdb5e63eee93 |
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| cites | cdi_FETCH-LOGICAL-c467t-927ffd5fabb985ecc11aed740082a84beef097db8baec5b480edcdb5e63eee93 |
| container_end_page | 154 |
| container_issue | 1 |
| container_start_page | 152 |
| container_title | Cryobiology |
| container_volume | 68 |
| creator | Niu, Hui-Ran Zi, Xiang-Dong Xiao, Xiao Xiong, Xian-Rong Zhong, Jin-Cheng Li, Jian Wang, Li Wang, Yong |
| description | In the present study, we examined the ability of immature germinal vesicle (GV) and subjected to in vitro matured (MII) yak oocytes to survive after cryopreservation as well as their subsequent development following in vitro maturation and fertilization. Both GV and MII oocytes were cryopreserved by using two different vitrification solutions (VS); VS-I contained 10% ethylene glycol (EG) and 10% dimethylsulfoxide (DMSO) in TCM-199+20% (v/v) fetal calf serum (FCS) whereas VS-II contained 40% EG+18% Ficoll+0.5M sucrose in TCM-199+20% FCS. The percentage of oocytes found to be morphologically normal was greater (P |
| doi_str_mv | 10.1016/j.cryobiol.2014.01.012 |
| format | article |
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Both GV and MII oocytes were cryopreserved by using two different vitrification solutions (VS); VS-I contained 10% ethylene glycol (EG) and 10% dimethylsulfoxide (DMSO) in TCM-199+20% (v/v) fetal calf serum (FCS) whereas VS-II contained 40% EG+18% Ficoll+0.5M sucrose in TCM-199+20% FCS. The percentage of oocytes found to be morphologically normal was greater (P<0.01) in VS-I group than in VS-II group. Rates of cleavage (30.6–42.2%) and blastocyst formation (2.9–8.9%) did not differ among groups, but were lower than in unfrozen control (55.7% and 25.4%, P<0.01). These results show that a combination of EG and DMSO or EG, Ficoll and sucrose can be used to cryopreserve yak oocytes in French straws.</description><identifier>ISSN: 0011-2240</identifier><identifier>EISSN: 1090-2392</identifier><identifier>DOI: 10.1016/j.cryobiol.2014.01.012</identifier><identifier>PMID: 24463092</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>Animals ; Blastocyst - cytology ; Blastocyst - drug effects ; Blastocyst - physiology ; Bos grunniens ; Cattle ; Cell Survival - drug effects ; Cryopreservation ; Cryopreservation - methods ; Cryoprotective Agents - pharmacology ; Culture Media ; Dimethyl Sulfoxide - pharmacology ; Embryo, Mammalian ; Embryonic Development - drug effects ; Ethylene Glycol - pharmacology ; Female ; Fertilization - drug effects ; Fertilization - physiology ; Fertilization in Vitro ; Ficoll - pharmacology ; In vitro development ; In vitro fertilization ; In vitro maturation ; Male ; Oocytes - cytology ; Oocytes - drug effects ; Oocytes - physiology ; Osmolar Concentration ; Sucrose - pharmacology ; Vitrification ; Yak oocytes</subject><ispartof>Cryobiology, 2014-02, Vol.68 (1), p.152-154</ispartof><rights>2014 Elsevier Inc.</rights><rights>Copyright © 2014 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c467t-927ffd5fabb985ecc11aed740082a84beef097db8baec5b480edcdb5e63eee93</citedby><cites>FETCH-LOGICAL-c467t-927ffd5fabb985ecc11aed740082a84beef097db8baec5b480edcdb5e63eee93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24463092$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Niu, Hui-Ran</creatorcontrib><creatorcontrib>Zi, Xiang-Dong</creatorcontrib><creatorcontrib>Xiao, Xiao</creatorcontrib><creatorcontrib>Xiong, Xian-Rong</creatorcontrib><creatorcontrib>Zhong, Jin-Cheng</creatorcontrib><creatorcontrib>Li, Jian</creatorcontrib><creatorcontrib>Wang, Li</creatorcontrib><creatorcontrib>Wang, Yong</creatorcontrib><title>Developmental competence of frozen-thawed yak (Bos grunniens) oocytes followed by in vitro maturation and fertilization</title><title>Cryobiology</title><addtitle>Cryobiology</addtitle><description>In the present study, we examined the ability of immature germinal vesicle (GV) and subjected to in vitro matured (MII) yak oocytes to survive after cryopreservation as well as their subsequent development following in vitro maturation and fertilization. Both GV and MII oocytes were cryopreserved by using two different vitrification solutions (VS); VS-I contained 10% ethylene glycol (EG) and 10% dimethylsulfoxide (DMSO) in TCM-199+20% (v/v) fetal calf serum (FCS) whereas VS-II contained 40% EG+18% Ficoll+0.5M sucrose in TCM-199+20% FCS. The percentage of oocytes found to be morphologically normal was greater (P<0.01) in VS-I group than in VS-II group. Rates of cleavage (30.6–42.2%) and blastocyst formation (2.9–8.9%) did not differ among groups, but were lower than in unfrozen control (55.7% and 25.4%, P<0.01). These results show that a combination of EG and DMSO or EG, Ficoll and sucrose can be used to cryopreserve yak oocytes in French straws.</description><subject>Animals</subject><subject>Blastocyst - cytology</subject><subject>Blastocyst - drug effects</subject><subject>Blastocyst - physiology</subject><subject>Bos grunniens</subject><subject>Cattle</subject><subject>Cell Survival - drug effects</subject><subject>Cryopreservation</subject><subject>Cryopreservation - methods</subject><subject>Cryoprotective Agents - pharmacology</subject><subject>Culture Media</subject><subject>Dimethyl Sulfoxide - pharmacology</subject><subject>Embryo, Mammalian</subject><subject>Embryonic Development - drug effects</subject><subject>Ethylene Glycol - pharmacology</subject><subject>Female</subject><subject>Fertilization - drug effects</subject><subject>Fertilization - physiology</subject><subject>Fertilization in Vitro</subject><subject>Ficoll - pharmacology</subject><subject>In vitro development</subject><subject>In vitro fertilization</subject><subject>In vitro maturation</subject><subject>Male</subject><subject>Oocytes - cytology</subject><subject>Oocytes - drug effects</subject><subject>Oocytes - physiology</subject><subject>Osmolar Concentration</subject><subject>Sucrose - pharmacology</subject><subject>Vitrification</subject><subject>Yak oocytes</subject><issn>0011-2240</issn><issn>1090-2392</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNqFkctuFDEQRS0EIsPAL0RehkUPZbf7tQPCU4rEJnvLjzJ46LYH2zNR5-vpZhK2kUoqqXSqSrqHkEsGOwasfbffmTRH7eO448DEDthS_BnZMBig4vXAn5MNAGMV5wIuyKuc9wDQdrV4SS64EG0NA9-Qu094wjEeJgxFjdTE6YAFg0EaHXUp3mOoyi91h5bO6je9-hgz_ZmOIXgM-S2N0cwFM3VxHOMK6Zn6QE--pEgnVY5JFR8DVcFSh6n40d__m7wmL5waM7556Fty--Xz7fW36ubH1-_XH24qI9quVAPvnLONU1oPfYPGMKbQdgKg56oXGtHB0Fnda4Wm0aIHtMbqBtsaEYd6S67OZw8p_jliLnLy2eA4qoDxmCVr60Es5-rmabQB1tf9mtyWtGfUpJhzQicPyU8qzZKBXPXIvXzUI1c9EthSfFm8fPhx1BPa_2uPPhbg_RnAJZOTxySz8asO6xOaIm30T_34C8ZYqGc</recordid><startdate>201402</startdate><enddate>201402</enddate><creator>Niu, Hui-Ran</creator><creator>Zi, Xiang-Dong</creator><creator>Xiao, Xiao</creator><creator>Xiong, Xian-Rong</creator><creator>Zhong, Jin-Cheng</creator><creator>Li, Jian</creator><creator>Wang, Li</creator><creator>Wang, Yong</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>201402</creationdate><title>Developmental competence of frozen-thawed yak (Bos grunniens) oocytes followed by in vitro maturation and fertilization</title><author>Niu, Hui-Ran ; Zi, Xiang-Dong ; Xiao, Xiao ; Xiong, Xian-Rong ; Zhong, Jin-Cheng ; Li, Jian ; Wang, Li ; Wang, Yong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c467t-927ffd5fabb985ecc11aed740082a84beef097db8baec5b480edcdb5e63eee93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Blastocyst - cytology</topic><topic>Blastocyst - drug effects</topic><topic>Blastocyst - physiology</topic><topic>Bos grunniens</topic><topic>Cattle</topic><topic>Cell Survival - drug effects</topic><topic>Cryopreservation</topic><topic>Cryopreservation - methods</topic><topic>Cryoprotective Agents - pharmacology</topic><topic>Culture Media</topic><topic>Dimethyl Sulfoxide - pharmacology</topic><topic>Embryo, Mammalian</topic><topic>Embryonic Development - drug effects</topic><topic>Ethylene Glycol - pharmacology</topic><topic>Female</topic><topic>Fertilization - drug effects</topic><topic>Fertilization - physiology</topic><topic>Fertilization in Vitro</topic><topic>Ficoll - pharmacology</topic><topic>In vitro development</topic><topic>In vitro fertilization</topic><topic>In vitro maturation</topic><topic>Male</topic><topic>Oocytes - cytology</topic><topic>Oocytes - drug effects</topic><topic>Oocytes - physiology</topic><topic>Osmolar Concentration</topic><topic>Sucrose - pharmacology</topic><topic>Vitrification</topic><topic>Yak oocytes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Niu, Hui-Ran</creatorcontrib><creatorcontrib>Zi, Xiang-Dong</creatorcontrib><creatorcontrib>Xiao, Xiao</creatorcontrib><creatorcontrib>Xiong, Xian-Rong</creatorcontrib><creatorcontrib>Zhong, Jin-Cheng</creatorcontrib><creatorcontrib>Li, Jian</creatorcontrib><creatorcontrib>Wang, Li</creatorcontrib><creatorcontrib>Wang, Yong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Cryobiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Niu, Hui-Ran</au><au>Zi, Xiang-Dong</au><au>Xiao, Xiao</au><au>Xiong, Xian-Rong</au><au>Zhong, Jin-Cheng</au><au>Li, Jian</au><au>Wang, Li</au><au>Wang, Yong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Developmental competence of frozen-thawed yak (Bos grunniens) oocytes followed by in vitro maturation and fertilization</atitle><jtitle>Cryobiology</jtitle><addtitle>Cryobiology</addtitle><date>2014-02</date><risdate>2014</risdate><volume>68</volume><issue>1</issue><spage>152</spage><epage>154</epage><pages>152-154</pages><issn>0011-2240</issn><eissn>1090-2392</eissn><abstract>In the present study, we examined the ability of immature germinal vesicle (GV) and subjected to in vitro matured (MII) yak oocytes to survive after cryopreservation as well as their subsequent development following in vitro maturation and fertilization. Both GV and MII oocytes were cryopreserved by using two different vitrification solutions (VS); VS-I contained 10% ethylene glycol (EG) and 10% dimethylsulfoxide (DMSO) in TCM-199+20% (v/v) fetal calf serum (FCS) whereas VS-II contained 40% EG+18% Ficoll+0.5M sucrose in TCM-199+20% FCS. The percentage of oocytes found to be morphologically normal was greater (P<0.01) in VS-I group than in VS-II group. Rates of cleavage (30.6–42.2%) and blastocyst formation (2.9–8.9%) did not differ among groups, but were lower than in unfrozen control (55.7% and 25.4%, P<0.01). These results show that a combination of EG and DMSO or EG, Ficoll and sucrose can be used to cryopreserve yak oocytes in French straws.</abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>24463092</pmid><doi>10.1016/j.cryobiol.2014.01.012</doi><tpages>3</tpages></addata></record> |
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| identifier | ISSN: 0011-2240 |
| ispartof | Cryobiology, 2014-02, Vol.68 (1), p.152-154 |
| issn | 0011-2240 1090-2392 |
| language | eng |
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| source | Elsevier |
| subjects | Animals Blastocyst - cytology Blastocyst - drug effects Blastocyst - physiology Bos grunniens Cattle Cell Survival - drug effects Cryopreservation Cryopreservation - methods Cryoprotective Agents - pharmacology Culture Media Dimethyl Sulfoxide - pharmacology Embryo, Mammalian Embryonic Development - drug effects Ethylene Glycol - pharmacology Female Fertilization - drug effects Fertilization - physiology Fertilization in Vitro Ficoll - pharmacology In vitro development In vitro fertilization In vitro maturation Male Oocytes - cytology Oocytes - drug effects Oocytes - physiology Osmolar Concentration Sucrose - pharmacology Vitrification Yak oocytes |
| title | Developmental competence of frozen-thawed yak (Bos grunniens) oocytes followed by in vitro maturation and fertilization |
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