Physiological characteristics underpinning successful cryopreservation of endemic and endangered species of Bromeliaceae from the Brazilian Atlantic Forest

The germination requirements and the basis of the optimal water content before and after cryopreservation were studied for ten endangered Brazilian species of Bromeliaceae. Constant and alternating temperature regimes were used to determine the best conditions for seed germination. The relationship...

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Bibliographic Details
Published in:Botanical journal of the Linnean Society 2014-12, Vol.176 (4), p.567-578
Main Authors: Rodrigues, Alba R. P, Forzza, Rafaela C, Andrade, Antonio C. S
Format: Article
Language:English
Subjects:
Bromeliaceae
cryopreservation
dormancy
endangered species
forests
freezing
germination temperature
orthodox seeds
relative humidity
seed drying
seed germination
seed storage behaviour
seeds
sorption
sorption isotherms
species reintroduction
storage time
temperature
threatened species
viability
vigor
water content
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Summary:The germination requirements and the basis of the optimal water content before and after cryopreservation were studied for ten endangered Brazilian species of Bromeliaceae. Constant and alternating temperature regimes were used to determine the best conditions for seed germination. The relationship between seed water content and relative humidity was evaluated using water sorption isotherms at 15 °C. Seeds were cryostored at four water contents (3, 5, 7 and 9%) and three storage periods (0, 180 and 365 days), and loss in viability and vigour were estimated. Fresh seeds of all species showed maximum germination in
ISSN:0024-4074
1095-8339
DOI:10.1111/boj.12219