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Alkaline phosphatases in microbialites and bacterioplankton from Alchichica soda lake, Mexico
Abstract Dissolved organic phosphorus utilization by different members of natural communities has been closely linked to microbial alkaline phosphatases whose affiliation and diversity is largely unknown. Here we assessed genetic diversity of bacterial alkaline phosphatases phoX and phoD, using high...
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Published in: | FEMS microbiology ecology 2014-11, Vol.90 (2), p.504-519 |
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description | Abstract
Dissolved organic phosphorus utilization by different members of natural communities has been closely linked to microbial alkaline phosphatases whose affiliation and diversity is largely unknown. Here we assessed genetic diversity of bacterial alkaline phosphatases phoX and phoD, using highly diverse microbial consortia (microbialites and bacterioplankton) as study models. These microbial consortia are found in an oligo-mesotrophic soda lake with a particular geochemistry, exhibiting a low calcium concentration and a high Mg : Ca ratio relative to seawater. In spite of the relative low calcium concentration in the studied system, our results highlight the diversity of calcium-based metallophosphatases phoX and phoD-like in heterotrophic bacteria of microbialites and bacterioplankton, where phoX was the most abundant alkaline phosphatase found. phoX and phoD-like phylotypes were more numerous in microbialites than in bacterioplankton. A larger potential community for DOP utilization in microbialites was consistent with the TN : TP ratio, suggesting P limitation within these assemblages. A cross-system comparison indicated that diversity of phoX in Lake Alchichica was similar to that of other aquatic systems with a naturally contrasting ionic composition and trophic state, although no phylotypes were shared among systems.
Bacterial assemblages of microbialites and bacterioplankton in the tropical soda lake Alchichica exhibit a vast potential for organic phosphorus utilization by calcium-dependent alkaline phosphatases.
Bacterial assemblages of microbialites and bacterioplankton in the tropical soda lake Alchichica exhibit a vast potential for organic phosphorus utilization by calcium-dependent alkaline phosphatases. |
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Dissolved organic phosphorus utilization by different members of natural communities has been closely linked to microbial alkaline phosphatases whose affiliation and diversity is largely unknown. Here we assessed genetic diversity of bacterial alkaline phosphatases phoX and phoD, using highly diverse microbial consortia (microbialites and bacterioplankton) as study models. These microbial consortia are found in an oligo-mesotrophic soda lake with a particular geochemistry, exhibiting a low calcium concentration and a high Mg : Ca ratio relative to seawater. In spite of the relative low calcium concentration in the studied system, our results highlight the diversity of calcium-based metallophosphatases phoX and phoD-like in heterotrophic bacteria of microbialites and bacterioplankton, where phoX was the most abundant alkaline phosphatase found. phoX and phoD-like phylotypes were more numerous in microbialites than in bacterioplankton. A larger potential community for DOP utilization in microbialites was consistent with the TN : TP ratio, suggesting P limitation within these assemblages. A cross-system comparison indicated that diversity of phoX in Lake Alchichica was similar to that of other aquatic systems with a naturally contrasting ionic composition and trophic state, although no phylotypes were shared among systems.
Bacterial assemblages of microbialites and bacterioplankton in the tropical soda lake Alchichica exhibit a vast potential for organic phosphorus utilization by calcium-dependent alkaline phosphatases.
Bacterial assemblages of microbialites and bacterioplankton in the tropical soda lake Alchichica exhibit a vast potential for organic phosphorus utilization by calcium-dependent alkaline phosphatases.</description><identifier>ISSN: 0168-6496</identifier><identifier>EISSN: 1574-6941</identifier><identifier>DOI: 10.1111/1574-6941.12411</identifier><identifier>PMID: 25112496</identifier><identifier>CODEN: FMECEZ</identifier><language>eng</language><publisher>England: Blackwell Publishing Ltd</publisher><subject>Alkaline Phosphatase - analysis ; Alkaline Phosphatase - genetics ; Alkaline Phosphatase - metabolism ; Aquatic environment ; Bacteria ; Bacteria - classification ; Bacteria - enzymology ; Bacteria - genetics ; Bacteria - isolation & purification ; Brackish ; Calcium ; Dissolved organic phosphorus ; dissolved organic phosphorus utilization ; Ecology ; extracellular enzymes ; Genetic diversity ; Genetic Variation ; Geochemistry ; Lakes ; Lakes - chemistry ; Lakes - microbiology ; low calcium environment ; Mexico ; microbial functional diversity ; Microbiology ; Molecular Sequence Data ; Phosphorus - metabolism ; Phylogeny ; Salinity ; Seawater</subject><ispartof>FEMS microbiology ecology, 2014-11, Vol.90 (2), p.504-519</ispartof><rights>2014 Federation of European Microbiological Societies. Published by John Wiley & Sons Ltd 2014</rights><rights>2014 Federation of European Microbiological Societies. Published by John Wiley & Sons Ltd. All rights reserved</rights><rights>2014 Federation of European Microbiological Societies. Published by John Wiley & Sons Ltd. All rights reserved.</rights><rights>Copyright © 2014 Federation of European Microbiological Societies. Published by John Wiley & Sons Ltd</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5501-bdd318bf086b9a96642d7feb2f4c9ef613b0e010657736a9f84f33f1d94a8ff63</citedby><cites>FETCH-LOGICAL-c5501-bdd318bf086b9a96642d7feb2f4c9ef613b0e010657736a9f84f33f1d94a8ff63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25112496$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Valdespino-Castillo, Patricia M.</creatorcontrib><creatorcontrib>Alcántara-Hernández, Rocio J.</creatorcontrib><creatorcontrib>Alcocer, Javier</creatorcontrib><creatorcontrib>Merino-Ibarra, Martín</creatorcontrib><creatorcontrib>Macek, Miroslav</creatorcontrib><creatorcontrib>Falcón, Luisa I.</creatorcontrib><title>Alkaline phosphatases in microbialites and bacterioplankton from Alchichica soda lake, Mexico</title><title>FEMS microbiology ecology</title><addtitle>FEMS Microbiol Ecol</addtitle><description>Abstract
Dissolved organic phosphorus utilization by different members of natural communities has been closely linked to microbial alkaline phosphatases whose affiliation and diversity is largely unknown. Here we assessed genetic diversity of bacterial alkaline phosphatases phoX and phoD, using highly diverse microbial consortia (microbialites and bacterioplankton) as study models. These microbial consortia are found in an oligo-mesotrophic soda lake with a particular geochemistry, exhibiting a low calcium concentration and a high Mg : Ca ratio relative to seawater. In spite of the relative low calcium concentration in the studied system, our results highlight the diversity of calcium-based metallophosphatases phoX and phoD-like in heterotrophic bacteria of microbialites and bacterioplankton, where phoX was the most abundant alkaline phosphatase found. phoX and phoD-like phylotypes were more numerous in microbialites than in bacterioplankton. A larger potential community for DOP utilization in microbialites was consistent with the TN : TP ratio, suggesting P limitation within these assemblages. A cross-system comparison indicated that diversity of phoX in Lake Alchichica was similar to that of other aquatic systems with a naturally contrasting ionic composition and trophic state, although no phylotypes were shared among systems.
Bacterial assemblages of microbialites and bacterioplankton in the tropical soda lake Alchichica exhibit a vast potential for organic phosphorus utilization by calcium-dependent alkaline phosphatases.
Bacterial assemblages of microbialites and bacterioplankton in the tropical soda lake Alchichica exhibit a vast potential for organic phosphorus utilization by calcium-dependent alkaline phosphatases.</description><subject>Alkaline Phosphatase - analysis</subject><subject>Alkaline Phosphatase - genetics</subject><subject>Alkaline Phosphatase - metabolism</subject><subject>Aquatic environment</subject><subject>Bacteria</subject><subject>Bacteria - classification</subject><subject>Bacteria - enzymology</subject><subject>Bacteria - genetics</subject><subject>Bacteria - isolation & purification</subject><subject>Brackish</subject><subject>Calcium</subject><subject>Dissolved organic phosphorus</subject><subject>dissolved organic phosphorus utilization</subject><subject>Ecology</subject><subject>extracellular enzymes</subject><subject>Genetic diversity</subject><subject>Genetic Variation</subject><subject>Geochemistry</subject><subject>Lakes</subject><subject>Lakes - chemistry</subject><subject>Lakes - microbiology</subject><subject>low calcium environment</subject><subject>Mexico</subject><subject>microbial functional diversity</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Phosphorus - metabolism</subject><subject>Phylogeny</subject><subject>Salinity</subject><subject>Seawater</subject><issn>0168-6496</issn><issn>1574-6941</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNqNkc1LxDAQxYMouq6evUnAi4hdM22aNsdlWT9A8aJHCWmbsHHbpjYt6n9v1q6LiKAhEJj83mNmHkJHQCbgzwXECQ0YpzCBkAJsodGmso1GBFgaMMrZHtp37pkQiCNKdtFeGIPnORuhp2m5lKWpFW4W1jUL2UmnHDY1rkze2sz4z84XZF3gTOadao1tSlkvO1tj3doKT8t8YVZXYmcLiUu5VOf4Tr2Z3B6gHS1Lpw7X7xg9Xs4fZtfB7f3VzWx6G-RxTCDIiiKCNNMkZRmXnDEaFolWWahpzpVmEGVEESAsTpKISa5TqqNIQ8GpTLVm0RidDr5Na1965TpRGZer0jeqbO8EsIhTzsMk_gcaJjxNSUo9evIDfbZ9W_tBPAXejZMw9NTFQPl1OdcqLZrWVLJ9F0DEKiSxikSsIhGfIXnF8dq3zypVbPivVDwQD8CrKdX7X37icn73ZXw26Gzf_KoKvnXxAcJ5ps8</recordid><startdate>201411</startdate><enddate>201411</enddate><creator>Valdespino-Castillo, Patricia M.</creator><creator>Alcántara-Hernández, Rocio J.</creator><creator>Alcocer, Javier</creator><creator>Merino-Ibarra, Martín</creator><creator>Macek, Miroslav</creator><creator>Falcón, Luisa I.</creator><general>Blackwell Publishing Ltd</general><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7QH</scope><scope>7UA</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>7X8</scope></search><sort><creationdate>201411</creationdate><title>Alkaline phosphatases in microbialites and bacterioplankton from Alchichica soda lake, Mexico</title><author>Valdespino-Castillo, Patricia M. ; Alcántara-Hernández, Rocio J. ; Alcocer, Javier ; Merino-Ibarra, Martín ; Macek, Miroslav ; Falcón, Luisa I.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5501-bdd318bf086b9a96642d7feb2f4c9ef613b0e010657736a9f84f33f1d94a8ff63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Alkaline Phosphatase - analysis</topic><topic>Alkaline Phosphatase - genetics</topic><topic>Alkaline Phosphatase - metabolism</topic><topic>Aquatic environment</topic><topic>Bacteria</topic><topic>Bacteria - classification</topic><topic>Bacteria - enzymology</topic><topic>Bacteria - genetics</topic><topic>Bacteria - isolation & purification</topic><topic>Brackish</topic><topic>Calcium</topic><topic>Dissolved organic phosphorus</topic><topic>dissolved organic phosphorus utilization</topic><topic>Ecology</topic><topic>extracellular enzymes</topic><topic>Genetic diversity</topic><topic>Genetic Variation</topic><topic>Geochemistry</topic><topic>Lakes</topic><topic>Lakes - chemistry</topic><topic>Lakes - microbiology</topic><topic>low calcium environment</topic><topic>Mexico</topic><topic>microbial functional diversity</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Phosphorus - metabolism</topic><topic>Phylogeny</topic><topic>Salinity</topic><topic>Seawater</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Valdespino-Castillo, Patricia M.</creatorcontrib><creatorcontrib>Alcántara-Hernández, Rocio J.</creatorcontrib><creatorcontrib>Alcocer, Javier</creatorcontrib><creatorcontrib>Merino-Ibarra, Martín</creatorcontrib><creatorcontrib>Macek, Miroslav</creatorcontrib><creatorcontrib>Falcón, Luisa I.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Aqualine</collection><collection>Water Resources Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS microbiology ecology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Valdespino-Castillo, Patricia M.</au><au>Alcántara-Hernández, Rocio J.</au><au>Alcocer, Javier</au><au>Merino-Ibarra, Martín</au><au>Macek, Miroslav</au><au>Falcón, Luisa I.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Alkaline phosphatases in microbialites and bacterioplankton from Alchichica soda lake, Mexico</atitle><jtitle>FEMS microbiology ecology</jtitle><addtitle>FEMS Microbiol Ecol</addtitle><date>2014-11</date><risdate>2014</risdate><volume>90</volume><issue>2</issue><spage>504</spage><epage>519</epage><pages>504-519</pages><issn>0168-6496</issn><eissn>1574-6941</eissn><coden>FMECEZ</coden><abstract>Abstract
Dissolved organic phosphorus utilization by different members of natural communities has been closely linked to microbial alkaline phosphatases whose affiliation and diversity is largely unknown. Here we assessed genetic diversity of bacterial alkaline phosphatases phoX and phoD, using highly diverse microbial consortia (microbialites and bacterioplankton) as study models. These microbial consortia are found in an oligo-mesotrophic soda lake with a particular geochemistry, exhibiting a low calcium concentration and a high Mg : Ca ratio relative to seawater. In spite of the relative low calcium concentration in the studied system, our results highlight the diversity of calcium-based metallophosphatases phoX and phoD-like in heterotrophic bacteria of microbialites and bacterioplankton, where phoX was the most abundant alkaline phosphatase found. phoX and phoD-like phylotypes were more numerous in microbialites than in bacterioplankton. A larger potential community for DOP utilization in microbialites was consistent with the TN : TP ratio, suggesting P limitation within these assemblages. A cross-system comparison indicated that diversity of phoX in Lake Alchichica was similar to that of other aquatic systems with a naturally contrasting ionic composition and trophic state, although no phylotypes were shared among systems.
Bacterial assemblages of microbialites and bacterioplankton in the tropical soda lake Alchichica exhibit a vast potential for organic phosphorus utilization by calcium-dependent alkaline phosphatases.
Bacterial assemblages of microbialites and bacterioplankton in the tropical soda lake Alchichica exhibit a vast potential for organic phosphorus utilization by calcium-dependent alkaline phosphatases.</abstract><cop>England</cop><pub>Blackwell Publishing Ltd</pub><pmid>25112496</pmid><doi>10.1111/1574-6941.12411</doi><tpages>16</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Alkaline Phosphatase - analysis Alkaline Phosphatase - genetics Alkaline Phosphatase - metabolism Aquatic environment Bacteria Bacteria - classification Bacteria - enzymology Bacteria - genetics Bacteria - isolation & purification Brackish Calcium Dissolved organic phosphorus dissolved organic phosphorus utilization Ecology extracellular enzymes Genetic diversity Genetic Variation Geochemistry Lakes Lakes - chemistry Lakes - microbiology low calcium environment Mexico microbial functional diversity Microbiology Molecular Sequence Data Phosphorus - metabolism Phylogeny Salinity Seawater |
title | Alkaline phosphatases in microbialites and bacterioplankton from Alchichica soda lake, Mexico |
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