Characterization of rhizobia homologues of Sinorhizobium meliloti insertion sequences ISRm3 and ISRm4

Homologues to Sinorhizobium meliloti insertion sequences ISRm3 and ISRm4 were found within Rhizobium leguminosarum bv. viciae field populations. Similarly, homologous sequences to S. meliloti ISRm4 were found in S. fredii strains. Based on the polymerase chain reaction, we cloned and partially seque...

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Bibliographic Details
Published in:FEMS microbiology ecology 1998-04, Vol.25 (4), p.341-348
Main Authors: Villadas, P.J, Burgos, P, Rodriquez-Navarro, D.N, Temprano, F, Toro, N
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Bacteriology
Biochemistry and biology
Biological and medical sciences
Chemical, physicochemical, biochemical and biological properties
comparisons
Ecology
Field release
Fundamental and applied biological sciences. Psychology
genbank/aj001622
genbank/aj001623
genbank/aj001624
genbank/x63715
genbank/x65471
genbank/x95567
Genetics
Homology
Insertion
Insertion sequence
Insertion sequences
ISRm3
ISRm4
ISRm6
Microbiology
molecular sequence data
Nucleotide sequence
nucleotide sequences
Physics, chemistry, biochemistry and biology of agricultural and forest soils
Polymerase chain reaction
Rhizobium leguminosarum
sequence homology
Sinorhizobium
Sinorhizobium meliloti
Soil science
transposons
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Summary:Homologues to Sinorhizobium meliloti insertion sequences ISRm3 and ISRm4 were found within Rhizobium leguminosarum bv. viciae field populations. Similarly, homologous sequences to S. meliloti ISRm4 were found in S. fredii strains. Based on the polymerase chain reaction, we cloned and partially sequenced some of these putative insertion sequence elements. DNA sequence comparisons indicate that S. meliloti and R. leguminosarum ISRm3-type elements are closely related whereas the ISRm4 homologues show distinct relationships. In addition, specific primers for PCR recognition of ISRm3 and ISRm4 elements in rhizobia species and S. meliloti ISRm6 were designed.
ISSN:0168-6496
1574-6941
DOI:10.1111/j.1574-6941.1998.tb00485.x