Expression of receptors for ovarian steroids and prostaglandin E2 in the endometrium and myometrium of mares during estrus, diestrus and early pregnancy

• Published in: Animal reproduction science 2014-12, Vol.151 (3-4), p.169-181
• Main Authors: Silva, E.S.M., Scoggin, K.E., Canisso, I.F., Troedsson, M.H.T., Squires, E.L., Ball, B.A.
• Format: Article
• Language: English
• Subjects: Animals; Diestrus - genetics; Diestrus - metabolism; Endometrium - metabolism; Equine; Estrous cycle; Estrous Cycle - genetics; Estrous Cycle - metabolism; Estrus - genetics; Estrus - metabolism; Female; Gestational Age; Gonadal Steroid Hormones - metabolism; Horses - genetics; Horses - metabolism; Myometrium - metabolism; Ovary - metabolism; Pregnancy; Pregnancy, Animal - genetics; Pregnancy, Animal - metabolism; Prostaglandin E2 receptor; Receptors, Prostaglandin E - genetics; Receptors, Prostaglandin E - metabolism; Receptors, Steroid - genetics; Receptors, Steroid - metabolism; Steroid receptor; Uterus
• ISSN: 0378-4320; 1873-2232
• DOI: 10.1016/j.anireprosci.2014.11.001

The objective of this study was to compare expression of estrogen receptor alpha (ER-α), β (ER-β), progesterone receptor (PR), as well as prostaglandin E2 type 2 (EP2) and 4 (EP4) receptors in the equine myometrium and endometrium during estrus, diestrus and early pregnancy. Tissues were collected during estrus, diestrus, and early pregnancy. Transcripts for ER-α (ESR1), ER-β (ESR2), PR (PGR), EP2 (PTGER2) and EP4 (PTGER4) were quantified by qPCR. Immunohistochemistry was used to localize ER-α, ER-β, PR, EP2 and EP4. Differences in transcript in endometrium and myometrium were compared by the ΔΔCT method. Expression for ESR1 (P