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Statistical experimental design to assess the influence of enzymes of nematophagous fungi versus helminths

•Plackett–Burman design was used to assess the destructive significance of enzymes.•We used Duddingtonia flagrans and Pochonia chlamydosporia proteases and chitinases.•All tested enzymes had a significant effect on the destruction of Strongyloides westeri larvae.•VC4 and AC001 proteases had a signif...

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Bibliographic Details
Published in:Research in veterinary science 2014-12, Vol.97 (3), p.527-532
Main Authors: Braga, Fabio Ribeiro, de Freitas Soares, Filippe Elias, Araujo, Juliana Milani, da Fonseca, Leandro Abreu, Hiura, Emy, Garschagen Gava, Maylla, Toledo Vieira, Fernanda, da Paz, Jeanne Saraiva, de Carvalho, Lorendane Milena, Faccini, João Victor, de Queiroz, José Humberto, Araújo, Jackson Victor
Format: Article
Language:English
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Summary:•Plackett–Burman design was used to assess the destructive significance of enzymes.•We used Duddingtonia flagrans and Pochonia chlamydosporia proteases and chitinases.•All tested enzymes had a significant effect on the destruction of Strongyloides westeri larvae.•VC4 and AC001 proteases had a significant effect on trichostrongylides larvae.•Chitinases of VC4 showed the highest significance on Platynosomum fastosum eggs destruction. The present work used Plackett–Burman experimental design to assess the influence of enzymes of nematophagous fungi versus Strongyloides westeri and trichostrongylides larvae and Platynosomum fastosum eggs. The variables studied in the Plackett–Burman design were the proteases and chitinases of AC001 or VC4 as destructive agents of S. westeri and trichostrongylides larvae, and P. fastosum eggs. All tested enzymes had a significant effect (P 
ISSN:0034-5288
1532-2661
DOI:10.1016/j.rvsc.2014.09.005