Liver immunolocation of CYP P450 in an subacute toxicity experiment with Clorpirifos o,o-(3, 5, 6- trichloride -2-pyridyl phosphorotioato in young male tilapia (Oreochromis spp)/Inmunolocalizacion hepatica de CYP P450 en un experimento de toxicidad subaguda por Clorpirifos 0,0-dietil 0-(3, 5, 6-tricloro-2-piridil fosforotioato) en machos juveniles de tilapia (Oreochromis spp)/Inmunolocalizacao hepatica de CYP P450 num teste de toxicidade subaguda por Clorpirifos 0,0-dietil 0-(3, 5, 6-tricloro-2-

• Published in: CES medicina veterinaria y zootecnia 2013-07, Vol.8 (2), p.61-61
• Main Authors: Chaparro, Jenny G, Taborda, Diego Alfonso Aranzazu, Urrea, Laura, Rodriguez, Berardo de J
• Format: Article
• Language: English
• Subjects: Hepatica; Oreochromis
• ISSN: 1900-9607; 1900-9607

In Colombia the diagnostic techniques for implementing immunohistochemical biomarkers to fulfill biomonitoring of environmental contamination are limited. The objective of this study is to implement an immunohistochemical technique to immunolocate CYP 1A in the tissue of fish, performing a subacute exposition experiment of Clofopirifos in the liver of young male tilapia. The sublethal dosage was carried out in semi-static state, with a daily refill of 50% of the water volume, maintaining the corresponding concentration in each group through the addition of half of the dosage until the 28th day. A significant difference for some liver lesions, confirming that it is an organ affected by low dosages of Cloropirifos. It verified that the induction of CYP 1A in hepatocyte, endothelium and biliary cells equally in exposed animals and those that weren't exposed. This study implemented and normalized an immunohistochemical technique for the tissue immunolocation of CYP 1A that could be used in future investigations to fulfill biomonitoring of contamination in Colombia's hydrographic basins.