Gene expression profiles of sodium-dependent vitamin C transporters in mice after alcohol consumption

Alcoholic liver disease （ALD） is a serious fiver problem in western countries. Our previous study has demonstrated that vitamin C plays a protective role in ALD. The vitamin C homeostasis is tightly regulated by sodium-dependent vitamin C transporters （SVCTs） 1 and 2. But the role of two SVCTs in AL...

Full description

Saved in:
Bibliographic Details
Published in:Acta biochimica et biophysica Sinica 2013-11, Vol.45 (11), p.912-920
Main Authors: Guo, Xiaoqiang, Wang, Yuejia, Shen, Yongqing, Gao, Yingjie, Chang, Yanzhong, Duan, Xianglin
Format: Article
Language:English
Subjects:
Alcohol Drinking
Animals
Ascorbic Acid - blood
Base Sequence
Blotting, Western
Chromatography, High Pressure Liquid
DNA Primers
Ethanol - toxicity
Gene Expression Profiling
Hypoxia-Inducible Factor 1, alpha Subunit - metabolism
Liver - drug effects
Liver - metabolism
Male
Mice
Real-Time Polymerase Chain Reaction
Sodium-Coupled Vitamin C Transporters - genetics
Up-Regulation
体内
基因表达谱
小鼠
维生素C含量
缺氧诱导因子
肠道吸收
转运体
饮酒
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Alcoholic liver disease （ALD） is a serious fiver problem in western countries. Our previous study has demonstrated that vitamin C plays a protective role in ALD. The vitamin C homeostasis is tightly regulated by sodium-dependent vitamin C transporters （SVCTs） 1 and 2. But the role of two SVCTs in ALD is less understood. In this study, we exam- ined the expression patterns of two SVCTs in mice after alcohol consumption. Our results suggested that alcohol con- sumption obviously increased the expression of two SVCTs in liver and SVCT1 in kidney and intestine, which is important for vitamin C absorption. Vitamin C supplement increased the sera vitamin C content and ameliorated the symptom of ALD. Intestinal absorption and renal re-absorption mediated by SVCTI are key factors to increase the sera vitamin C content after alcohol consumption. We proposed that both reactive oxygen species and low vitamin C concentration regulate the expression of SVCTs, and the protective role of vitamin C is mediated by suppressing the stability of hypoxia-inducible factor-loL. Thus, our study is significant for the understanding of vitamin C homeostasis in ALD and for better use of other antioxidants in ALD therapy.
ISSN:1672-9145
1745-7270
DOI:10.1093/abbs/gmt099