Isolation and selection of cellulolytic fungi from palm oil mill effluent

Cellulolytic fungi, 34 strains, were isolated from samples taken from palm oil mill residues and effluent, and high cellulase producers selected in comparison with nine known reference strains. Although 13 isolates showed good filter paper distintegration within 14 days, only eight isolates exhibite...

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Bibliographic Details
Published in:World journal of microbiology & biotechnology 1992-11, Vol.8 (6), p.614-617
Main Authors: Prasertsan, P, H-Kittikul, A, Chitmanee, B
Format: Article
Language:English
Subjects:
Aspergillus
Aspergillus niger
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
biosynthesis
Biotechnology
cellulose
cellulose digestion
Elaeis guineensis
enzyme activity
Fundamental and applied biological sciences. Psychology
Hypocrea jecorina
Hypocrea rufa
isolation
Isolation and description
Mission oriented research
Myrothecium verrucaria
palm oil mill effluent
Phanerochaete chrysosporium
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Summary:Cellulolytic fungi, 34 strains, were isolated from samples taken from palm oil mill residues and effluent, and high cellulase producers selected in comparison with nine known reference strains. Although 13 isolates showed good filter paper distintegration within 14 days, only eight isolates exhibited clearing zones around their colonies on carboxymethylcellulose (CMC) agar medium. Quantitative cellulase activity measurements, using CMC as carbon source, selected three of the eight isolates as potential cellulase producers. Using dried palm oil mill condensate as carbon source, only one of the isolates (F 11) showed similar results on both carbon sources. During media optimization for CMCase production, a four-fold increase from 0.058 to 0.275 U/ml was obtained using a medium, containing 0.1% (v/v) Tween 80 0.02% (w/v) NH4NO3, 0.025% (w/v) proteose-peptone and 0.1% (w/v) CMC dissolved in undiluted condensate from the sterilization of oil palm bunches, with an initial pH of 5.5.
ISSN:0959-3993
1573-0972
DOI:10.1007/bf01238799